ArticleThe Journal of cell biology1991
Kinetic analysis of F-actin depolymerization in polymorphonuclear leukocyte lysates indicates that chemoattractant stimulation increases actin filament number without altering the filament length distribution.
Article in The Journal of cell biology, 1991. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 45 papers.
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Who cites it
45 citing papers in PubMed, 128 citations in OpenAlex.
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- Sex-Based Differences in Human Neutrophil Chemorepulsion.Journal of immunology (Baltimore, Md. : 1950) · 2022Article
- Measuring expression heterogeneity of single-cell cytoskeletal protein complexes.Nature communications · 2021Article
- The role of mode switching in a population of actin polymers with constraints.Journal of mathematical biology · 2021Article
- Toward a new picture of the living plasma membrane.Protein science : a publication of the Protein Society · 2020Review
- Multicompartment cell-based modeling of confined migration: regulation by cell intrinsic and extrinsic factors.Molecular biology of the cell · 2018Article
- Akt3 kinase suppresses pinocytosis of low-density lipoprotein by macrophages via a novel WNK/SGK1/Cdc42 protein pathway.The Journal of biological chemistry · 2017Article
- Mechanochemical Signaling Directs Cell-Shape Change.Biophysical journal · 2017Review
- GPI-anchored protein organization and dynamics at the cell surface.Journal of lipid research · 2016Review
- Nuclear envelope composition determines the ability of neutrophil-type cells to passage through micron-scale constrictions.The Journal of biological chemistry · 2013Article
- Non-muscle myosin II induces disassembly of actin stress fibres independently of myosin light chain dephosphorylation.Interface focus · 2011Article
- Measurements of spatiotemporal changes in G-actin concentration reveal its effect on stimulus-induced actin assembly and lamellipodium extension.The Journal of cell biology · 2011Article
- Response of an actin filament network model under cyclic stretching through a coarse grained Monte Carlo approach.Journal of theoretical biology · 2011Article
- LIM kinases are required for invasive path generation by tumor and tumor-associated stromal cells.The Journal of cell biology · 2010Article
- Active transport and cluster formation on 2D networks.The European physical journal. E, Soft matter · 2010Article
- A numerical model of cellular blebbing: a volume-conserving, fluid-structure interaction model of the entire cell.Journal of biomechanics · 2010Article
- Mammalian CARMIL inhibits actin filament capping by capping protein.Developmental cell · 2005Article
- The mechanics of neutrophils: synthetic modeling of three experiments.Biophysical journal · 2003Article
- Modulatory role of focal adhesion kinase in regulating human pulmonary arterial endothelial barrier function.The Journal of physiology · 2002Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
The rate of filamentous actin (F-actin) depolymerization is proportional to the number of filaments depolarizing and changes in the rate are proportional to changes in filament number. To determine the number and length of actin filaments in polymorphonuclear leukocytes and the change in filament number and length that occurs during the increase in F-actin upon chemoattractant stimulation, the time course of cellular F-actin depolymerization in lysates of control and peptide-stimulated cells was examined. F-actin was quantified by the TRITC-labeled phalloidin staining of pelletable actin. Lysis in 1.2 M KCl and 10 microM DNase I minimized the effects of F-actin binding proteins and G-actin, respectively, on the kinetics of depolymerization. To determine filament number and length from a depolymerization time course, depolymerization kinetics must be limited by the actin monomer dissociation rate. Comparison of time courses of depolymerization in the presence (pointed ends free) or absence (barbed and pointed ends free) of cytochalasin suggested depolymerization occurred from both ends of the filament and that monomer dissociation was rate limiting. Control cells had 1.7 +/- 0.4 x 10(5) filaments with an average length of 0.29 +/- 0.09 microns. Chemo-attractant stimulation for 90 s at room temperature with 0.02 microM N-formylnorleucylleucylphenylalanine caused a twofold increase in F-actin and about a two-fold increase in the total number of actin filaments to 4.0 +/- 0.5 x 10(5) filaments with an average length of 0.27 +/- 0.07 microns. In both cases, most (approximately 80%) of the filaments were quite short (less than or equal to 0.18 micron). The length distributions of actin filaments in stimulated and control cells were similar.
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