ArticleBirth defects research. Part A, Clinical and molecular teratology2009
Regulation of folate receptor 1 gene expression in the visceral endoderm.
Article in Birth defects research. Part A, Clinical and molecular teratology, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed, 24 citations in OpenAlex.
- Analyzing Co-expression Networks with Network Skeleton Extraction.Research square · 2026Article
- Review
- Aberrant lipid accumulation in the mouse visceral yolk sac resulting from maternal diabetes and obesity.Frontiers in cell and developmental biology · 2023Article
- Association of periconceptional folate supplements andZhong nan da xue xue bao. Yi xue ban = Journal of Central South University. Medical sciences · 2022Article
- Nutrient Transporter Gene Expression in the Early Conceptus-Implications From Two Mouse Models of Diabetic Pregnancy.Frontiers in cell and developmental biology · 2022Article
- Folic Acid Exposure Rescues Spina Bifida Aperta Phenotypes in Human Induced Pluripotent Stem Cell Model.Scientific reports · 2018Article
- Regulation of Reduced Folate Carrier (RFC) by Vitamin D Receptor at the Blood-Brain Barrier.Molecular pharmaceutics · 2017Article
- Modeling anterior development in mice: diet as modulator of risk for neural tube defects.American journal of medical genetics. Part C, Seminars in medical genetics · 2013Review
- Article
- Genetic and epigenomic footprints of folate.Progress in molecular biology and translational science · 2012Review
- Diabetic embryopathy: a role for the epigenome?Birth defects research. Part A, Clinical and molecular teratology · 2011Review
- Folic acid in early pregnancy: a public health success story.FASEB journal : official publication of the Federation of American Societies for Experimental Biology · 2010Article
- Androgen activation of the folate receptor α gene through partial tethering of the androgen receptor by C/EBPα.The Journal of steroid biochemistry and molecular biology · 2010Article
Corrections and comments
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Authors and funding
3 authors at 2 institutions in 1 country.
Funding
Abstract
backgroundNutrient supply to the developing mammalian embryo is a fundamental requirement. Before completion of the chorioallantoic placenta, the visceral endoderm plays a crucial role in nurturing the embryo. We have found that visceral endoderm cells express folate receptor 1, a high-affinity receptor for the essential micronutrient folic acid, suggesting that the visceral endoderm has an important function for folate transport to the embryo. The mechanisms that direct expression of FOLR1 in the visceral endoderm are unknown.
methodsSequences were tested for transcriptional activation capabilities in the visceral endoderm utilizing reporter gene assays in a cell model for extraembryonic endoderm in vitro, and in transgenic mice in vivo.
resultsWith F9 embryo carcinoma cells as a model for extraembryonic endoderm, we demonstrate that the P4 promoter of the human FOLR1 gene is active during differentiation of the cells towards visceral endoderm. However, transgenic mouse experiments show that promoter sequences alone are insufficient to elicit reporter gene transcription in vivo. Using sequence conservation as guide to choose genomic sequences from the human FOLR1 gene locus, we demonstrate that the sequence termed F1CE2 exhibits specific enhancer activity in F9 cells in vitro, in the visceral endoderm, and later the yolk sac in transgenic mouse embryos in vivo. We further show that the transcription factor HNF4-alpha can activate this enhancer sequence.
conclusionsWe have identified a transcriptional enhancer sequence from the FOLR1 locus with specific activity in vitro and in vivo, and suggest that FOLR1 is a target for regulation by HNF4-alpha.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.