ArticleNature protocols2009
The preparation and development of cellular membrane affinity chromatography columns.
Article in Nature protocols, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 33 papers.
What it found
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Who cites it
33 citing papers in PubMed.
- Emodin and physcion alleviate cholestatic liver injury by targeting FXR: hepatoprotective components identified in processedFrontiers in pharmacology · 2025Article
- Overcoming the On-Target Toxicity in Antibody-Mediated Therapies via an Indirect Active Targeting Strategy.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2023Article
- High-Resolution Bioassay Profiling with Complemented Sensitivity and Resolution for Pancreatic Lipase Inhibitor Screening.Molecules (Basel, Switzerland) · 2022Article
- Article
- Cellular Membrane Affinity Chromatography Columns to Identify Specialized Plant Metabolites Interacting with Immobilized Tropomyosin Kinase Receptor B.Journal of visualized experiments : JoVE · 2022Article
- Recent advances in screening active components from natural products based on bioaffinity techniques.Acta pharmaceutica Sinica. B · 2020Review
- Analysis of solute-protein interactions and solute-solute competition by zonal elution affinity chromatography.Methods (San Diego, Calif.) · 2018Review
- Analysis of stereoselective drug interactions with serum proteins by high-performance affinity chromatography: A historical perspective.Journal of pharmaceutical and biomedical analysis · 2017Review
- Analysis of Biological Interactions by Affinity Chromatography: Clinical and Pharmaceutical Applications.Clinical chemistry · 2017Review
- Comparison of analytical techniques for the identification of bioactive compounds from natural products.Natural product reports · 2016Review
- Targeting Anti-Cancer Active Compounds: Affinity-Based Chromatographic Assays.Current pharmaceutical design · 2016Review
- The development of mitochondrial membrane affinity chromatography columns for the study of mitochondrial transmembrane proteins.Analytical biochemistry · 2015Article
- Highly stabilized, polymer-lipid membranes prepared on silica microparticles as stationary phases for capillary chromatography.Journal of chromatography. A · 2015Article
- Multiple protein stationary phases: a review.Journal of chromatography. B, Analytical technologies in the biomedical and life sciences · 2014Review
- Analysis of biomolecular interactions using affinity microcolumns: a review.Journal of chromatography. B, Analytical technologies in the biomedical and life sciences · 2014Review
- Resveratrol and its metabolites bind to PPARs.Chembiochem : a European journal of chemical biology · 2014Article
- Comparative normal/failing rat myocardium cell membrane chromatographic analysis system for screening specific components that counteract doxorubicin-induced heart failure from Acontium carmichaeli.Analytical chemistry · 2014Article
- Characterization of a multiple endogenously expressed adenosine triphosphate-binding cassette transporters using nuclear and cellular membrane affinity chromatography columns.Journal of chromatography. A · 2014Article
- Identification of metabolites of propyrisulfuron in rats.Journal of chromatography. B, Analytical technologies in the biomedical and life sciences · 2014Article
- Stabilized phospholipid membranes in chromatography: toward membrane protein-functionalized stationary phases.Analytical and bioanalytical chemistry · 2014Article
Corrections and comments
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Authors and funding
2 authors.
Funding
Abstract
Cellular membrane affinity chromatography is a technique that is based on the immobilization of a target trans-membrane protein onto a stationary phase. The target protein is isolated by homogenization and solubilization of a source (e.g., cell line) followed by immobilization on either the immobilized artificial membrane-phosphatidyl choline (IAM-PC) stationary phase or the surface of an open tubular capillary during a dialysis step. The procedure typically takes 3-4 d for the IAM-PC stationary phase, whereas the open-tubular method takes an extra week for the preparation of the capillary. The resulting columns can then be used to characterize binding sites on the target protein through frontal chromatographic and/or nonlinear chromatographic studies using a wide variety of ligands including small molecules and polypeptides. The columns have been used in drug discovery as well as in the screening of tobacco smoke condensates.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.