ArticleNature protocols2009
Protocol for the quantitative assessment of DNA concentration and damage (fragmentation and nicks).
Article in Nature protocols, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed.
- Novel intravenous formulation for radiosensitization in osteosarcoma treatment.Materials today. Bio · 2025Article
- Development of Sensitive Methods for the Detection of Minimum Concentrations of DNA on Martian Soil Simulants.Life (Basel, Switzerland) · 2023Article
- Short tandem repeat (STR) instability in the oral mucosa of patients submitted to fixed orthodontic therapy: a limitation of STR profile quality for human identification.Forensic science, medicine, and pathology · 2022Article
- Diclofenac Administration after Physical Training Blunts Adaptations of Peripheral Systems and Leads to Losses in Exercise Performance: In Vivo and In Silico Analyses.Antioxidants (Basel, Switzerland) · 2021Article
- Parasporin A13-2 ofToxins · 2021Article
- A universal fluorescence-based toolkit for real-time quantification of DNA and RNA nuclease activity.Scientific reports · 2019Article
- Protein carbonyl determination by a rhodamine B hydrazide-based fluorometric assay.Redox biology · 2018Article
- Regulation of Tumor Progression by Programmed Necrosis.Oxidative medicine and cellular longevity · 2018Review
- DNA damage in dental pulp mesenchymal stem cells: AnVeterinary research forum : an international quarterly journal · 2018Article
- Oxidative Stress, DNA, Cell Cycle/Cell Cycle Associated Proteins and Multidrug Resistance Proteins: Targets of Human Amniotic Membrane in Hepatocellular Carcinoma.Pathology oncology research : POR · 2016Article
- A new platform for ultra-high density Staphylococcus aureus transposon libraries.BMC genomics · 2015Article
- Biopsies: next-generation biospecimens for tailoring therapy.Nature reviews. Clinical oncology · 2013Review
- O-hydroxylamine-coupled alkaline gel electrophoresis assay for the detection and measurement of DNA single-strand breaks.Methods in molecular biology (Clifton, N.J.) · 2012Article
- Accumulation of true single strand breaks and AP sites in base excision repair deficient cells.Mutation research · 2010Article
Corrections and comments
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Authors and funding
3 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
An ultrasensitive protocol is presented for the quantitative assessment of fragmented and nicked dsDNA using PicoGreen and consists of four methods. The first quantifies the concentration of DNA, whereas the second (quantitative complement of the Comet assay) quantifies the degree of DNA fragmentation seen in a typical DNA agarose electrophoresis gel. Both methods have sensitivity of 5 pg of DNA. The third method (quantitative counterpart of the electrophoresis-based qualitative apoptotic and necrotic DNA assays) quantifies the polyethylene glycol-fractionated small-size (0-1 kb) fragmented DNA. This method also detects up to 5 pg of damaged DNA and requires a minimum sample of quantity 0.2 ml of 2.5 microg ml(-1). The fourth method measures the percentage of DNA nicks by alkaline DNA unwinding and requires up to 15 pg of DNA sample. The time required for processing 10 DNA samples is 1/2, 1, 13 and 1 h for the first, second, third and fourth method, respectively.
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Registered trials
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