ArticleAmerican journal of physiology. Gastrointestinal and liver physiology2009
Very high aquaporin-1 facilitated water permeability in mouse gallbladder.
Article in American journal of physiology. Gastrointestinal and liver physiology, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
What it found
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Who cites it
20 citing papers in PubMed, 38 citations in OpenAlex.
- Aquaporin membrane channels in the hepatobiliary tract: a model of complexity and clinical implications in health and disease.Internal and emergency medicine · 2026Review
- Aquaporins in Biliary Function: Pathophysiological Implications and Therapeutic Targeting.International journal of molecular sciences · 2024Review
- Altered Cell Clusters and Upregulated Aqp1 in Connexin 50 Knockout Lens Epithelium.Investigative ophthalmology & visual science · 2024Article
- Article
- Review
- AQP3-mediated activation of the AMPK/SIRT1 signaling pathway curtails gallstone formation in mice by inhibiting inflammatory injury of gallbladder mucosal epithelial cells.Molecular medicine (Cambridge, Mass.) · 2023Article
- Aquaporins in Glandular Secretion.Advances in experimental medicine and biology · 2023Article
- Hepatobiliary Thyroid Hormone Deficiency Impacts Bile Acid Hydrophilicity and Aquaporins in Cholestatic C57BL/6J Mice.International journal of molecular sciences · 2022Article
- Physiological and pathological impact of AQP1 knockout in mice.Bioscience reports · 2019Review
- Microfluidic platform for rapid measurement of transepithelial water transport.Lab on a chip · 2017Article
- Retrospective analysis of canine gallbladder contents in biliary sludge and gallbladder mucoceles.The Journal of veterinary medical science · 2017Article
- Phosphorylation and subcellular localization of NaJournal of physiology and biochemistry · 2017Article
- Aquaporin gene therapy for disorders of cholestasis?Hepatology (Baltimore, Md.) · 2016Article
- Aquaporins in Health and Disease: An Overview Focusing on the Gut of Different Species.International journal of molecular sciences · 2016Review
- Rapid Identification of Novel Inhibitors of the Human Aquaporin-1 Water Channel.Chemical biology & drug design · 2016Article
- Aquaporin-1 Deficiency Protects Against Myocardial Infarction by Reducing Both Edema and Apoptosis in Mice.Scientific reports · 2015Article
- Cholesterol gallstone disease: focusing on the role of gallbladder.Laboratory investigation; a journal of technical methods and pathology · 2015Review
- The aquaporin 1 C-terminal tail is required for migration and growth of pulmonary arterial myocytes.American journal of respiratory cell and molecular biology · 2014Article
- Detection of volume changes in calcein-stained cells using confocal microscopy.Journal of fluorescence · 2013Article
- Aquaporin water channels in transepithelial fluid transport.The journal of medical investigation : JMI · 2009Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
Water transport across gallbladder epithelium is driven by osmotic gradients generated from active salt absorption and secretion. Aquaporin (AQP) water channels have been proposed to facilitate transepithelial water transport in gallbladder and to modulate bile composition. We found strong AQP1 immunofluorescence at the apical membrane of mouse gallbladder epithelium. Transepithelial osmotic water permeability (Pf) was measured in freshly isolated gallbladder sacs from the kinetics of luminal calcein self-quenching in response to an osmotic gradient. Pf was very high (0.12 cm/s) in gallbladders from wild-type mice, cAMP independent, and independent of osmotic gradient size and direction. Although gallbladders from AQP1 knockout mice had similar size and morphology to those from wild-type mice, their Pf was reduced by approximately 10-fold. Apical plasma membrane water permeability was greatly reduced in AQP1-deficient gallbladders, as measured by cytoplasmic calcein quenching in perfluorocarbon-filled, inverted gallbladder sacs. However, neither bile osmolality nor bile salt concentration differed in gallbladders from wild-type vs. AQP1 knockout mice. Our data indicate constitutively high water permeability in mouse gallbladder epithelium involving transcellular water transport through AQP1. The similar bile salt concentration in gallbladders from AQP1 knockout mice argues against a physiologically important role for AQP1 in mouse gallbladder.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.