ArticleInfection and immunity2009
The CsgA and Lpp proteins of an Escherichia coli O157:H7 strain affect HEp-2 cell invasion, motility, and biofilm formation.
Article in Infection and immunity, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers.
What it found
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Who cites it
28 citing papers in PubMed, 58 citations in OpenAlex.
- Peptidoglycan LD-Transpeptidases.Antibiotics (Basel, Switzerland) · 2025Review
- Article
- No Genomic Signatures Were Found inVeterinary sciences · 2025Article
- Lipoprotein Lpp and L, D-transpeptidases regulate the master regulator of virulence AggR in EAEC.Scientific reports · 2025Article
- Pathogenicity and host-interacting mechanisms of enterogenicFrontiers in microbiology · 2025Article
- Central metabolism is a key player in E. coli biofilm stimulation by sub-MIC antibiotics.PLoS genetics · 2023Article
- Characterization of functional amyloid curli in biofilm formation of an environmental isolate Enterobacter cloacae SBP-8.Antonie van Leeuwenhoek · 2023Article
- Article
- Control of Escherichia coli Serotype O157:H7 Motility and Biofilm Formation by Salicylate and Decanoate: MarA/SoxS/Rob andApplied and environmental microbiology · 2022Article
- Antibacterial Efficacy of Liposomal Formulations Containing Tobramycin andPharmaceutics · 2022Article
- Article
- Emergence of a Synergistic Diversity as a Response to Competition in Pseudomonas putida Biofilms.Microbial ecology · 2020Article
- Interplay between Peptidoglycan Biology and Virulence in Gram-Negative Pathogens.Microbiology and molecular biology reviews : MMBR · 2018Review
- Pch Genes Control Biofilm and Cell Adhesion in a Clinical Serotype O157:H7 Isolate.Frontiers in microbiology · 2018Article
- Absence of Curli in Soil-PersistentFrontiers in microbiology · 2018Article
- Curli Temper Adherence of Escherichia coli O157:H7 to Squamous Epithelial Cells from the Bovine Recto-Anal Junction in a Strain-Dependent Manner.Applied and environmental microbiology · 2017Article
- The virulence factor ychO has a pleiotropic action in an Avian Pathogenic Escherichia coli (APEC) strain.BMC microbiology · 2016Article
- Effects of Selected Egyptian Honeys on the Cellular Ultrastructure and the Gene Expression Profile of Escherichia coli.PloS one · 2016Article
- Expression of Curli byFrontiers in cellular and infection microbiology · 2016Article
- Article
Corrections and comments
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Authors and funding
4 authors at 4 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
In Escherichia coli O157:H7 strain ATCC 43895, a guanine-to-thymine transversion in the csgD promoter created strain 43895OR. Strain 43895OR produces an abundant extracellular matrix rich in curli fibers, forms biofilms on solid surfaces, invades cultured epithelial cells, and is more virulent in mice than strain 43895. In this study we compared the formic acid-soluble proteins expressed by strains 43895OR and 43895 using one-dimensional sodium dodecyl sulfate-polyacrylamide gel electrophoresis analysis and identified two differentially expressed proteins. A 17-kDa protein unique to strain 43895OR was identified from matrix-assisted laser desorption ionization-time of flight analysis combined with mass spectrometry (MS) and tandem MS (MS/MS) as the curli subunit encoded by csgA. A <10-kDa protein, more highly expressed in strain 43895, was identified as the Lpp lipoprotein. Mutants of strain 43895OR with disruption of lpp, csgA, or both lpp and csgA were created and tested for changes in phenotype and function. The results of this study show that both Lpp and CsgA contribute to the observed colony morphology, Congo red binding, motility, and biofilm formation. We also show that both CsgA and Lpp are required by strain 43895OR for the invasion of cultured HEp-2 cells. These studies suggest that in strain 43895OR, the murein lipoprotein Lpp indirectly regulates CsgA expression through the CpxAR system by a posttranscriptional mechanism.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.