ArticleEpigenetics & chromatin2009
High-resolution mapping of heterochromatin redistribution in a Drosophila position-effect variegation model.
Article in Epigenetics & chromatin, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 29 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
29 citing papers in PubMed, 52 citations in OpenAlex.
- Review
- The Evolution of Locally Adaptive Chromosome Inversions in Mimulus guttatus.Molecular ecology · 2025Article
- Article
- The impact of cell states on heterochromatin dynamics.The Biochemical journal · 2024Review
- Article
- Species-specific chromatin landscape determines how transposable elements shape genome evolution.eLife · 2022Article
- Live analysis of position-effect variegation inProceedings of the National Academy of Sciences of the United States of America · 2022Article
- Exact breakpoints of themicroPublication biology · 2022Article
- Fine-Scale Position Effects Shape the Distribution of Inversion Breakpoints in Drosophila melanogaster.Genome biology and evolution · 2020Article
- Double-edged sword: The evolutionary consequences of the epigenetic silencing of transposable elements.PLoS genetics · 2020Review
- Evolution and Diversification ofFrontiers in plant science · 2019Article
- (Epi)genetic Inheritance in Schistosoma mansoni: A Systems Approach.Trends in parasitology · 2017Review
- Generalized nucleation and looping model for epigenetic memory of histone modifications.Proceedings of the National Academy of Sciences of the United States of America · 2016Article
- Inducible DamID systems for genomic mapping of chromatin proteins in Drosophila.Nucleic acids research · 2016Article
- Targeting of P-Element Reporters to Heterochromatic Domains by Transposable Element 1360 in Drosophila melanogaster.Genetics · 2016Article
- The Differences Between Cis- and Trans-Gene Inactivation Caused by Heterochromatin in Drosophila.Genetics · 2016Article
- Human inversions and their functional consequences.Briefings in functional genomics · 2015Review
- Position-effect variegation, heterochromatin formation, and gene silencing in Drosophila.Cold Spring Harbor perspectives in biology · 2013Review
- Maternal depletion of Piwi, a component of the RNAi system, impacts heterochromatin formation in Drosophila.PLoS genetics · 2013Article
- Chromatin: constructing the big picture.The EMBO journal · 2011Review
Corrections and comments
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Authors and funding
6 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundPosition-effect variegation (PEV) is the stochastic transcriptional silencing of a gene positioned adjacent to heterochromatin. white-mottled X-chromosomal inversions in Drosophila are classic PEV models that show variegation of the eye color gene white due to its relocation next to pericentric heterochromatin. It has been suggested that in these models the spreading of heterochromatin across the rearrangement breakpoint causes the silencing of white. However, the extent of this spreading and the precise pattern of heterochromatin redistribution have remained unclear. To obtain insight into the mechanism of PEV, we constructed high-resolution binding maps of Heterochromatin Protein 1 (HP1) on white-mottled chromosomes.
resultsWe find that HP1 invades euchromatin across the inversion breakpoints over approximately 175 kb and approximately 30 kb, causing de novo association of HP1 with 20 genes. However, HP1 binding levels in these regions show substantial local variation, and white is the most strongly bound gene. Remarkably, white is also the only gene that is detectably repressed by heterochromatin. Furthermore, we find that HP1 binding to the invaded region is particularly sensitive to the dosage of the histone methyltransferase Su(var)3-9, indicating that the de novo formed heterochromatin is less stable than naturally occurring constitutive heterochromatin.
conclusionOur molecular maps demonstrate that heterochromatin can invade a normally euchromatic region, yet the strength of HP1 binding and effects on gene expression are highly dependent on local context. Our data suggest that the white gene has an unusual intrinsic affinity for heterochromatin, which may cause this gene to be more sensitive to PEV than most other genes.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.