ArticleAnalytical chemistry2009
High-sensitivity nanoLC-MS/MS analysis of urinary desmosine and isodesmosine.
Article in Analytical chemistry, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
9 citing papers in PubMed, 54 citations in OpenAlex.
- Isotope-dilution LC-MS/MS analysis of the elastin crosslinkers desmosine and isodesmosine in acute cerebral stroke patients.RSC advances · 2022Article
- Decreased humoral immune response in the bronchi of rapid decliners with chronic obstructive pulmonary disease.Respiratory research · 2022Article
- Quantification of desmosine and isodesmosine using MALDI-ion trap tandem mass spectrometry.Analytical and bioanalytical chemistry · 2018Article
- Free Urinary Desmosine and Isodesmosine as COPD Biomarkers: The Relevance of Confounding Factors.Chronic obstructive pulmonary diseases (Miami, Fla.) · 2016Article
- Fingerprinting desmosine-containing elastin peptides.Journal of the American Society for Mass Spectrometry · 2015Article
- Electron transfer and collision induced dissociation of non-derivatized and derivatized desmosine and isodesmosine.Journal of the American Society for Mass Spectrometry · 2013Article
- Glycoproteomics using fluid-based specimens in the discovery of lung cancer protein biomarkers: promise and challenge.Proteomics. Clinical applications · 2013Review
- Multiple liquid chromatography separations and nanoESI-ion trap detection of plasma proteins in search of stroke biomarkers: A pilot study.Journal of separation science · 2012Article
- Cation exchange HPLC analysis of desmosines in elastin hydrolysates.Analytical and bioanalytical chemistry · 2011Article
Corrections and comments
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Authors and funding
8 authors at 3 institutions in 2 countries.
Funding
Abstract
Chronic obstructive pulmonary disease (COPD) is characterized by the degradation of elastin, the major insoluble protein of lung tissues. The degradation of elastin gives rise to desmosine (DES) and isodesmosine (IDES), two major urinary products typified by a hydrophilic pyridinium-based cross-linker structure. A high sensitivity method based on nanoflow liquid chromatography tandem mass spectrometry with multiple reaction monitoring was developed for the analysis of urinary DES and IDES. The analytes were derivatized with propionic anhydride and deuterated DES (D(4)-DES) was used as an internal standard. This method enables the quantification of DES and IDES in as little as 50 microL of urine and provides a detection limit of 0.10 ng/mL (0.95 fmol on-column). We report the analysis of DES and IDES in a cohort of 40 urine specimens from four groups of individuals: (a) COPD rapid decliners (11.8 +/- 3.7 ng/mg creatine (crea)), (b) COPD slow decliners (16.0 +/- 3.1 ng/mg crea), (c) healthy smokers (13.2 +/- 1.9 ng/mg crea), and (d) healthy nonsmokers (14.9 +/- 2.9 ng/mg crea). Our analysis reveals a statistically significant decrease in the level of urinary DES and IDES in COPD rapid decliner patients compared to healthy nonsmoker controls and COPD slow decliner patients. This methodology may be useful for monitoring DES and IDES levels in well controlled animal models for COPD or for longitudinal studies in COPD patients.
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Registered trials
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