ArticleIn vitro cellular & developmental biology : journal of the Tissue Culture Association1991
Isolation of two morphologically distinct cell lines from rat arterial smooth muscle expressing high tumorigenic potentials.
Article in In vitro cellular & developmental biology : journal of the Tissue Culture Association, 1991. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.
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Who cites it
9 citing papers in PubMed, 38 citations in OpenAlex.
- Induction of microRNA-1 by myocardin in smooth muscle cells inhibits cell proliferation.Arteriosclerosis, thrombosis, and vascular biology · 2011Article
- Phenotype-specific inhibition of the vascular smooth muscle cell cycle by high glucose treatment.Diabetologia · 2007Article
- 6A3-5/Osa2 is an early activated gene implicated in the control of vascular smooth muscle cell functions.Journal of biomedicine & biotechnology · 2006Article
- Identification, structural, and functional characterization of a new early gene (6A3-5, 7 kb): implication in the proliferation and differentiation of smooth muscle cells.Journal of biomedicine & biotechnology · 2005Article
- The reversal of the inhibition on lipids synthesis by L-659,699 in arterial smooth muscle cells cultures.Molecular and cellular biochemistry · 2001Article
- Vessel size-dependent expression of intermediate-sized filaments, calponin, and h-caldesmon in smooth muscle cells of human coronary arteries.Heart and vessels · 1999Article
- Changes in cultured arterial smooth muscle cells isolated from chicks upon cholesterol feeding.Lipids · 1998Article
- Lipid biosynthesis in cultured arterial smooth muscle cells is related to their phenotype.Lipids · 1993Article
- Ring formation in cultures of rat aortic smooth muscle cells.In vitro cellular & developmental biology : journal of the Tissue Culture AssociationArticle
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Authors and funding
6 authors at 4 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Smooth muscle cell proliferation is an important feature of atherogenesis. Some works have hypothesized that a transformation of smooth muscle cells could arise during this pathological process. The present paper describes two spontaneously transformed cell lines of arterial smooth muscle cells (SMC) established from aortic media of adult rat. The cell lines have been designated V6 and V8; some of their morphologic, growth, and metabolic characteristics are described and compared to their parent cells. The two cell lines appeared distinct by their morphology and by their degree of transformation. V6 cells appeared as elongated spindle-shaped cells whereas V8 cells were spread cells with a cobblestone pattern. Karyotypes of both cell lines showed a high polyploidy level. V6 and V8 cell lines were immortalized and showed growth characteristics of transformed cells: low requirement of serum to grow, ability to form colonies in soft agar and tumorigenicity in nude mice; V8 cells presented a higher malignancy than V6 cells. Both V6 and V8 cells exhibited characteristics of cultured arterial SMC: ultrastructure, alpha actin expression at the protein and mRNA level, prostacyclin production. The remarkably different morphologies of the V6 and V8 lines and their transformed phenotype suggest that these cell lines could be useful models to study SMC differentiation and proliferation with respect to atherosclerotic or hypertensive vascular diseases.
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