ArticleProtein science : a publication of the Protein Society2009
Parallel screening and optimization of protein constructs for structural studies.
Article in Protein science : a publication of the Protein Society, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.
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Who cites it
1 citing paper in PubMed.
- A systematic mutagenesis-driven strategy for site-resolved NMR studies of supramolecular assemblies.Journal of biomolecular NMR · 2011Article
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Authors and funding
10 authors.
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Abstract
A major challenge in structural biology remains the identification of protein constructs amenable to structural characterization. Here, we present a simple method for parallel expression, labeling, and purification of protein constructs (up to 80 kDa) combined with rapid evaluation by NMR spectroscopy. Our approach, which is equally applicable for manual or automated implementation, offers an efficient way to identify and optimize protein constructs for NMR or X-ray crystallographic investigations.
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