ArticleThe Journal of investigative dermatology2009
Wnt5A activates the calpain-mediated cleavage of filamin A.
Article in The Journal of investigative dermatology, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 39 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
39 citing papers in PubMed, 71 citations in OpenAlex.
- Review
- Role of the Ror family receptors in Wnt5a signaling.In vitro cellular & developmental biology. Animal · 2024Review
- The Role of Calcium Signaling in Melanoma.International journal of molecular sciences · 2022Review
- Inducible Depletion of Calpain-2 Mitigates Abdominal Aortic Aneurysm in Mice.Arteriosclerosis, thrombosis, and vascular biology · 2021Article
- RYK-mediated filopodial pathfinding facilitates midgut elongation.Development (Cambridge, England) · 2020Article
- The Canonical Wnt Signaling (Wnt/β-Catenin Pathway): A Potential Target for Cancer Prevention and Therapy.Iranian biomedical journal · 2020Review
- Noncanonical WNT Activation in Human Right Ventricular Heart Failure.Frontiers in cardiovascular medicine · 2020Article
- ATG5 Mediates a Positive Feedback Loop between Wnt Signaling and Autophagy in Melanoma.Cancer research · 2017Article
- Wnt signaling in cancer.Oncogene · 2017Review
- Ror2 signaling regulates Golgi structure and transport through IFT20 for tumor invasiveness.Scientific reports · 2017Article
- Signaling regulation and role of filamin A cleavage in Ca2+-stimulated migration of androgen receptor-deficient prostate cancer cells.Oncotarget · 2017Article
- Wnt5a Signaling in Cancer.Cancers · 2016Review
- WNT-5A: signaling and functions in health and disease.Cellular and molecular life sciences : CMLS · 2016Review
- Article
- Activation of β2-adrenergic receptor by (R,R')-4'-methoxy-1-naphthylfenoterol inhibits proliferation and motility of melanoma cells.Cellular signalling · 2015Article
- Wnt5A promotes an adaptive, senescent-like stress response, while continuing to drive invasion in melanoma cells.Pigment cell & melanoma research · 2015Article
- Biologic and clinical significance of androgen receptor variants in castration resistant prostate cancer.Endocrine-related cancer · 2014Review
- Functional interaction with filamin A and intracellular Ca2+ enhance the surface membrane expression of a small-conductance Ca2+-activated K+ (SK2) channel.Proceedings of the National Academy of Sciences of the United States of America · 2014Article
- WNT5A-mediated β-catenin-independent signalling is a novel regulator of cancer cell metabolism.Carcinogenesis · 2014Article
- Hypoxia-induced and calpain-dependent cleavage of filamin A regulates the hypoxic response.Proceedings of the National Academy of Sciences of the United States of America · 2014Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
14 authors at 3 institutions in 2 countries.
Funding
Abstract
We have previously shown that Wnt5A and ROR2, an orphan tyrosine kinase receptor, interact to mediate melanoma cell motility. In other cell types, this can occur through the interaction of ROR2 with the cytoskeletal protein filamin A. Here, we found that filamin A protein levels correlated with Wnt5A levels in melanoma cells. Small interfering RNA (siRNA) knockdown of WNT5A decreased filamin A expression. Knockdown of filamin A also corresponded to a decrease in melanoma cell motility. In metastatic cells, filamin A expression was predominant in the cytoplasm, which western analysis indicated was due to the cleavage of filamin A in these cells. Treatment of nonmetastatic melanoma cells with recombinant Wnt5A increased filamin A cleavage, and this could be prevented by the knockdown of ROR2 expression. Further, BAPTA-AM chelation of intracellular calcium also inhibited filamin A cleavage, leading to the hypothesis that Wnt5A/ROR2 signaling could cleave filamin A through activation of calcium-activated proteases, such as calpains. Indeed, WNT5A knockdown decreased calpain 1 expression, and by inhibiting calpain 1 either pharmacologically or using siRNA, it decreased cell motility. Our results indicate that Wnt5A activates calpain-1, leading to the cleavage of filamin A, which results in a remodeling of the cytoskeleton and an increase in melanoma cell motility.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.