ArticleCell cycle (Georgetown, Tex.)2009
Subnuclear compartmentalization of transiently expressed polyadenylated pri-microRNAs: processing at transcription sites or accumulation in SC35 foci.
Article in Cell cycle (Georgetown, Tex.), 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
18 citing papers in PubMed, 24 citations in OpenAlex.
- An NF90/NF110-mediated feedback amplification loop regulates dicer expression and controls ovarian carcinoma progression.Cell research · 2018Article
- NEAT1 scaffolds RNA-binding proteins and the Microprocessor to globally enhance pri-miRNA processing.Nature structural & molecular biology · 2017Article
- miRNA Enriched in Human Neuroblast Nuclei Bind the MAZ Transcription Factor and Their Precursors Contain the MAZ Consensus Motif.Frontiers in molecular neuroscience · 2017Article
- HP1BP3, a Chromatin Retention Factor for Co-transcriptional MicroRNA Processing.Molecular cell · 2016Article
- Primary microRNA processing is functionally coupled to RNAP II transcription in vitro.Scientific reports · 2015Article
- Article
- The prolyl isomerase Pin1 targets stem-loop binding protein (SLBP) to dissociate the SLBP-histone mRNA complex linking histone mRNA decay with SLBP ubiquitination.Molecular and cellular biology · 2012Article
- RNA-related nuclear functions of human Pat1b, the P-body mRNA decay factor.Molecular biology of the cell · 2012Article
- A precursor microRNA in a cancer cell nucleus: get me out of here!Cell cycle (Georgetown, Tex.) · 2011Article
- LIN-28 co-transcriptionally binds primary let-7 to regulate miRNA maturation in Caenorhabditis elegans.Nature structural & molecular biology · 2011Article
- Integration of expressed sequence tag data flanking predicted RNA secondary structures facilitates novel non-coding RNA discovery.PloS one · 2011Article
- A role for TREX components in the release of spliced mRNA from nuclear speckle domains.Nature communications · 2010Article
- Nuclear networking fashions pre-messenger RNA and primary microRNA transcripts for function.Trends in cell biology · 2010Review
- Integrative analysis of the human cis-antisense gene pairs, miRNAs and their transcription regulation patterns.Nucleic acids research · 2010Article
- Coupled RNA processing and transcription of intergenic primary microRNAs.Molecular and cellular biology · 2009Article
- An evolutionary perspective of animal microRNAs and their targets.Journal of biomedicine & biotechnology · 2009Review
- MicroRNA-biogenesis and Pre-mRNA splicing crosstalk.Journal of biomedicine & biotechnology · 2009Review
- MicroRNA biogenesis: regulating the regulators.Critical reviews in biochemistry and molecular biologyReview
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
MicroRNAs (miRNAs) are small, noncoding RNAs that post-transcriptionally regulate expression of their target messenger RNAs. We recently demonstrated that primary miRNA transcripts (pri-miRNAs) retained at transcription sites are processed with enhanced efficiency, suggesting that pri-miRNA processing is coupled to transcription in mammalian cells. We also observed that transiently expressed pri-miRNAs accumulate in nuclear foci with splicing factor SC35 and Microprocessor components, Drosha and DGCR8. Here, we show that pri-miRNAs containing a self-cleaving hepatitis delta ribozyme accumulate in the nucleoplasm after release from their transcription sites, but are not efficiently processed. Pri-miRNAs with ribozyme-generated 3' ends do not localize to SC35-containing foci, whereas cleaved and polyadenylated pri-miRNA transcripts with or without the pre-miRNA hairpin do. Pri-miRNA/SC35 foci contain a number of proteins normally associated with SC35 domains, including ASF/SF2, PABII, and the prolyl isomerase, Pin1. In contrast, RNA polymerase II and PM/Scl-100 do not strongly colocalize with pri-miRNAs in SC35-containing foci. These data argue that pri-miRNA/SC35-containing foci are not major sites of pri-miRNA processing and that pri-miRNA processing is coupled to transcription. We discuss the implications of our findings relative to recent insights into miRNA biogenesis, mRNA metabolism, and the nuclear organization of gene expression.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.