ArticleThe Journal of neuroscience : the official journal of the Society for Neuroscience2009
Sustained in vivo inhibition of protein domains using single-chain Fv recombinant antibodies and its application to dissect RGMa activity on axonal outgrowth.
Article in The Journal of neuroscience : the official journal of the Society for Neuroscience, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 7 papers.
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Who cites it
7 citing papers in PubMed.
- Single chain fragment variable, a new theranostic approach for cardiovascular diseases.Frontiers in immunology · 2024Review
- An antibody to RGMa promotes regeneration of cochlear synapses after noise exposure.Scientific reports · 2021Article
- RGMs: Structural Insights, Molecular Regulation, and Downstream Signaling.Trends in cell biology · 2017Review
- ϒ-secretase and LARG mediate distinct RGMa activities to control appropriate layer targeting within the optic tectum.Cell death and differentiation · 2016Article
- CRMP-2 is involved in axon growth inhibition induced by RGMa in vitro and in vivo.Molecular neurobiology · 2013Article
- Novel roles of the chemorepellent axon guidance molecule RGMa in cell migration and adhesion.Molecular and cellular biology · 2012Article
- RGMA and neogenin protein expression are influenced by lens injury following optic nerve crush in the rat retina.Graefe's archive for clinical and experimental ophthalmology = Albrecht von Graefes Archiv fur klinische und experimentelle Ophthalmologie · 2012Article
Corrections and comments
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Authors and funding
4 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Antibodies are powerful tools for delineating the specific function of protein domains, yet several limitations restrict their in vivo applicability. Here we present a new method to obtain sustained in vivo inhibition of specific protein domains using recombinant antibodies. We show that long term in vivo expression of single-chain Fv (scFv) fragments in the developing CNS can be achieved through retroviral transduction. Moreover, specific scFvs generated against the N- and C-terminal domains of the repulsive guidance molecule, RGMa, prevent proper axon targeting in the visual system. This work reveals a previously unappreciated role for the RGMa N-terminal domain in axon guidance, and provides a novel, broadly applicable and rapid procedure to functionally antagonize any protein domain in vivo.
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Registered trials
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