ArticleRNA (New York, N.Y.)2009
Impact of normalization on miRNA microarray expression profiling.
Article in RNA (New York, N.Y.), 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 98 papers.
What it found
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Who cites it
98 citing papers in PubMed, 173 citations in OpenAlex.
- MicroRNA profiling in plasma samples using qPCR arrays: Recommendations for correct analysis and interpretation.PloS one · 2018Trial
- MicroRNA expression profiles as biomarkers of minor salivary gland inflammation and dysfunction in Sjögren's syndrome.Arthritis and rheumatism · 2011Trial
- Signature of immune infiltration-related ferroptosis genes to predict the prognosis of patients with osteosarcoma.Oncology letters · 2025Article
- Discovering promising drug candidates for Parkinson's disease: integrating miRNA and DEG analysis with molecular dynamics and MMPBSA.Journal of computer-aided molecular design · 2025Article
- MicroRNAs implicated in canine diffuse large B-cell lymphoma prognosis.FEBS open bio · 2024Article
- Uncovering miRNA-mRNA Regulatory Networks Related to Olaparib Resistance and Resensitization ofCells · 2024Article
- Exosomal MicroRNA as Biomarkers for Diagnosing or Monitoring the Progression of Ovarian Clear Cell Carcinoma: A Pilot Study.Molecules (Basel, Switzerland) · 2022Article
- Association of Androgenic Regulation and MicroRNAs in Acinar Adenocarcinoma of Prostate.Genes · 2022Article
- Regulatory effects of miR-28 on osteogenic differentiation of human bone marrow mesenchymal stem cells.Bioengineered · 2022Article
- Integrated analysis of miRNAome transcriptome and degradome reveals miRNA-target modules governing floral florescence development and senescence across early- and late-flowering genotypes in tree peony.Frontiers in plant science · 2022Article
- MicroRNA characteristics in epithelial ovarian cancer.PloS one · 2021Article
- A Post-Processing Algorithm for miRNA Microarray Data.International journal of molecular sciences · 2020Article
- Diurnal Patterns of Gene Expression in the Dorsal Vagal Complex and the Central Nucleus of the Amygdala - Non-rhythm-generating Brain Regions.Frontiers in neuroscience · 2020Article
- Micro-RNAs secreted through astrocyte-derived extracellular vesicles cause neuronal network degeneration in C9orf72 ALS.EBioMedicine · 2019Article
- Long-term impact of maternal high-fat diet on offspring cardiac health: role of micro-RNA biogenesis.Cell death discovery · 2019Article
- Normalization Methods for the Analysis of Unbalanced Transcriptome Data: A Review.Frontiers in bioengineering and biotechnology · 2019Review
- Circulating microRNAs as potential diagnostic biomarkers for osteoporosis.Scientific reports · 2018Article
- Detection of high-grade neoplasia in air-dried cervical PAP smears by a microRNA-based classifier.Oncology reports · 2018Article
- Article
- Identification of Serum MicroRNAs as Novel Biomarkers in Esophageal Squamous Cell Carcinoma Using Feature Selection Algorithms.Frontiers in oncology · 2018Article
38 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
8 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Profiling miRNA levels in cells with miRNA microarrays is becoming a widely used technique. Although normalization methods for mRNA gene expression arrays are well established, miRNA array normalization has so far not been investigated in detail. In this study we investigate the impact of normalization on data generated with the Agilent miRNA array platform. We have developed a method to select nonchanging miRNAs (invariants) and use them to compute linear regression normalization coefficients or variance stabilizing normalization (VSN) parameters. We compared the invariants normalization to normalization by scaling, quantile, and VSN with default parameters as well as to no normalization using samples with strong differential expression of miRNAs (heart-brain comparison) and samples where only a few miRNAs are affected (by p53 overexpression in squamous carcinoma cells versus control). All normalization methods performed better than no normalization. Normalization procedures based on the set of invariants and quantile were the most robust over all experimental conditions tested. Our method of invariant selection and normalization is not limited to Agilent miRNA arrays and can be applied to other data sets including those from one color miRNA microarray platforms, focused gene expression arrays, and gene expression analysis using quantitative PCR.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.