ArticleThe Journal of antimicrobial chemotherapy2009
Altered sterol profile induced in Leishmania amazonensis by a natural dihydroxymethoxylated chalcone.
Article in The Journal of antimicrobial chemotherapy, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed.
- Sterol Endoperoxides and Their Antileishmanial Effects: Influence on Viability, Oxygen Metabolism and Sterol Synthesis.Molecules (Basel, Switzerland) · 2026Article
- Chalcone Derivative Induces Flagellar Disruption and Autophagic Phenotype inPathogens (Basel, Switzerland) · 2023Article
- Biological effects ofFrontiers in cellular and infection microbiology · 2023Article
- In vitro leishmanicidal activity of two cholesterol derivatives.World journal of microbiology & biotechnology · 2022Article
- Simvastatin Resistance ofMicroorganisms · 2022Article
- Unexpected Role of Sterol Synthesis in RNA Stability and Translation inBiomedicines · 2021Article
- The effects of trans-chalcone and chalcone 4 hydrate on the growth of Babesia and Theileria.PLoS neglected tropical diseases · 2019Article
- Lipidomics and anti-trypanosomatid chemotherapy.Clinical and translational medicine · 2017Review
- Polymeric Nanoparticles of Brazilian Red Propolis Extract: Preparation, Characterization, Antioxidant and Leishmanicidal Activity.Nanoscale research letters · 2016Article
- Antileishmanial Activity of Ezetimibe: Inhibition of Sterol Biosynthesis, In Vitro Synergy with Azoles, and Efficacy in Experimental Cutaneous Leishmaniasis.Antimicrobial agents and chemotherapy · 2016Article
- In Vitro Antileishmanial Activity of Sterols from Trametes versicolor (Bres. Rivarden).Molecules (Basel, Switzerland) · 2016Article
- Dynamics of sterol synthesis during development of Leishmania spp. parasites to their virulent form.Parasites & vectors · 2016Article
- Imipramine alters the sterol profile in Leishmania amazonensis and increases its sensitivity to miconazole.Parasites & vectors · 2016Article
- Attenuation of Leishmania infantum chagasi metacyclic promastigotes by sterol depletion.Infection and immunity · 2013Article
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Authors and funding
7 authors.
Funding
Abstract
objectivesThe effects of the antileishmanial chalcone 2',6'-dihydroxy-4'-methoxychalcone (DMC) on Leishmania amazonensis sterol composition and biosynthesis were investigated to obtain information about the mechanism of growth inhibition by DMC on this parasite.
methodsThe interference of sterol biosynthesis by DMC was studied in drug-treated promastigotes by two different methods. (i) Newly synthesized sterols from parasites grown in the presence of [(3)H]mevalonate were analysed by thin layer chromatography (TLC)/fluorography. (ii) Total sterols extracted from the parasites grown with or without DMC were characterized by gas chromatography coupled to mass spectroscopy (GC/MS).
resultsTLC and GC/MS analyses of sterols extracted from DMC-treated promastigotes revealed the accumulation of early precursors and a reduction in the levels of C-14 demethylated and C-24 alkylated sterols, as well as a reduction in exogenous cholesterol uptake.
conclusionsThis study demonstrates that the natural chalcone DMC alters the sterol composition of L. amazonensis and suggests that the parasite target is different from other known sterol inhibitors.
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