ArticleBioinformatics (Oxford, England)2009
Serial dilution curve: a new method for analysis of reverse phase protein array data.
Article in Bioinformatics (Oxford, England), 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 31 papers.
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Who cites it
31 citing papers in PubMed.
- Tissue-Specific Downregulation of Fatty Acid Synthase Suppresses Intestinal Adenoma Formation via Coordinated Reprograming of Transcriptome and Metabolism in the Mouse Model of Apc-Driven Colorectal Cancer.International journal of molecular sciences · 2022Article
- Characterization of anticancer drug resistance by reverse-phase protein array: new targets and strategies.Expert review of proteomics · 2022Review
- Reverse phase protein arrays in acute leukemia: investigative and methodological challenges.Expert review of proteomics · 2021Review
- Multiomics analysis of serial PARP inhibitor treated metastatic TNBC inform on rational combination therapies.NPJ precision oncology · 2021Article
- Four-parameter paired response curve for serial dilution assays.Journal of biopharmaceutical statistics · 2021Article
- Integrative analysis of multi-platform reverse-phase protein array data for the pharmacodynamic assessment of response to targeted therapies.Scientific reports · 2020Article
- Mst1/2 kinases restrain transformation in a novel transgenic model of Ras driven non-small cell lung cancer.Oncogene · 2020Article
- Flavivirus Cross-Reactivity to Dengue Nonstructural Protein 1 Antigen Detection Assays.Diagnostics (Basel, Switzerland) · 2019Article
- Article
- Spectral Clustering via sparse graph structure learning with application to Proteomic Signaling Networks in Cancer.Computational statistics & data analysis · 2019Article
- Preclinical evaluation and reverse phase protein Array-based profiling of PI3K and MEK inhibitors in endometrial carcinoma in vitro.BMC cancer · 2018Article
- Article
- Integrated Genomic Characterization of Pancreatic Ductal Adenocarcinoma.Cancer cell · 2017Article
- Integrative Analysis Identifies Four Molecular and Clinical Subsets in Uveal Melanoma.Cancer cell · 2017Article
- Statistical Contributions to Bioinformatics: Design, Modeling, Structure Learning, and Integration.Statistical modelling · 2017Article
- Analysis of Reverse Phase Protein Array Data: From Experimental Design towards Targeted Biomarker Discovery.Microarrays (Basel, Switzerland) · 2015Review
- ATM may be a protective factor in endometrial carcinogenesis with the progesterone pathway.Tumour biology : the journal of the International Society for Oncodevelopmental Biology and Medicine · 2015Article
- Cupid: simultaneous reconstruction of microRNA-target and ceRNA networks.Genome research · 2015Article
- Microarray R-based analysis of complex lysate experiments with MIRACLE.Bioinformatics (Oxford, England) · 2014Article
- PIK3CA mutations in androgen receptor-positive triple negative breast cancer confer sensitivity to the combination of PI3K and androgen receptor inhibitors.Breast cancer research : BCR · 2014Article
Corrections and comments
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Authors and funding
6 authors.
Funding
Abstract
unlabelledReverse phase protein arrays (RPPAs) are a powerful high-throughput tool for measuring protein concentrations in a large number of samples. In RPPA technology, the original samples are often diluted successively multiple times, forming dilution series to extend the dynamic range of the measurements and to increase confidence in quantitation. An RPPA experiment is equivalent to running multiple ELISA assays concurrently except that there is usually no known protein concentration from which one can construct a standard response curve. Here, we describe a new method called 'serial dilution curve for RPPA data analysis'. Compared with the existing methods, the new method has the advantage of using fewer parameters and offering a simple way of visualizing the raw data. We showed how the method can be used to examine data quality and to obtain robust quantification of protein concentrations. AVAILABILITY: A computer program in R for using serial dilution curve for RPPA data analysis is freely available at http://odin.mdacc.tmc.edu/~zhangli/RPPA.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.