ArticleThe Journal of biological chemistry2009
The Carboxyl-terminal Domain of RNA Polymerase II Is Not Sufficient to Enhance the Efficiency of Pre-mRNA Capping or Splicing in the Context of a Different Polymerase.
Article in The Journal of biological chemistry, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
19 citing papers in PubMed, 34 citations in OpenAlex.
- Structure of a transcribing Pol II-DSIF-SPT6-U1 snRNP complex.Nature communications · 2025Article
- Directed evolution of an orthogonal transcription engine for programmable gene expression in eukaryotes.iScience · 2025Article
- Directed evolution of an orthogonal transcription engine for programmable gene expression in eukaryotes.bioRxiv : the preprint server for biology · 2024Article
- Evolution of the Early Spliceosomal Complex-From Constitutive to Regulated Splicing.International journal of molecular sciences · 2021Review
- Interplay of mRNA capping and transcription machineries.Bioscience reports · 2020Review
- C3P3-G1: first generation of a eukaryotic artificial cytoplasmic expression system.Nucleic acids research · 2019Article
- Versatility of Cyclophilins in Plant Growth and Survival: A Case Study inBiomolecules · 2019Review
- Pre-mRNA Splicing in the Nuclear Landscape.Cold Spring Harbor symposia on quantitative biology · 2019Article
- Identification of RNA targets for the nuclear multidomain cyclophilin atCyp59 and their effect on PPIase activity.Nucleic acids research · 2013Article
- Co-transcriptional regulation of alternative pre-mRNA splicing.Biochimica et biophysica acta · 2012Review
- Intron cleavage affects processing of alternatively spliced transcripts.RNA (New York, N.Y.) · 2011Article
- A novel splice variant in the N-propeptide of COL5A1 causes an EDS phenotype with severe kyphoscoliosis and eye involvement.PloS one · 2011Article
- A dual interface determines the recognition of RNA polymerase II by RNA capping enzyme.The Journal of biological chemistry · 2010Article
- Nuclear networking fashions pre-messenger RNA and primary microRNA transcripts for function.Trends in cell biology · 2010Review
- "Cotranscriptionality": the transcription elongation complex as a nexus for nuclear transactions.Molecular cell · 2009Review
- Co-transcriptional splicing of constitutive and alternative exons.RNA (New York, N.Y.) · 2009Article
- Towards T7 RNA polymerase (T7RNAP)-based expression system in yeast: challenges and opportunities.BioengineeredReview
- Pre-mRNA splicing during transcription in the mammalian system.Wiley interdisciplinary reviews. RNAReview
- Transcription and splicing: when the twain meet.TranscriptionArticle
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Authors and funding
3 authors at 3 institutions in 1 country.
Funding
Abstract
Eukaryotic messenger RNA precursors (pre-mRNAs) synthesized by RNA polymerase II (RNAP II) are processed co-transcriptionally. The carboxyl-terminal domain (CTD) of the largest subunit of RNA polymerase II is thought to mediate the coupling of transcription with pre-mRNA processing by coordinating the recruitment of processing factors during synthesis of nascent transcripts. Previous studies have demonstrated that the phosphorylated CTD is required for efficient co-transcriptional processing. In the study presented here we investigated whether the CTD is sufficient to coordinate transcription with pre-mRNA capping and splicing in the context of two other DNA-dependent RNA polymerases, mammalian RNAP III and bacteriophage T7 RNAP. Our results indicate that the CTD fused to the largest subunit of RNAP III (POLR3A) is not sufficient to enhance co-transcriptional pre-mRNA splicing or capping in vivo. Additionally, we analyzed a T7 RNAP-CTD fusion protein and examined its ability to enhance pre-mRNA splicing and capping of both constitutively and alternatively spliced substrates. We observed that the CTD in the context of T7 RNAP was not sufficient to enhance pre-mRNA splicing or capping either in vitro or in vivo. We propose that the efficient coupling of transcription to pre-mRNA processing requires not only the phosphorylated CTD but also other RNAP II specific subunits or associated factors.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.