ArticleThe Journal of biological chemistry2009
G Protein Mono-ubiquitination by the Rsp5 Ubiquitin Ligase.
Article in The Journal of biological chemistry, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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Who cites it
22 citing papers in PubMed.
- Protein post-translational modifications in serine synthetic pathway: functions and molecular mechanisms.Cell communication and signaling : CCS · 2025Review
- Role of K63-linked ubiquitination in cancer.Cell death discovery · 2022Review
- Emerging Roles of Non-proteolytic Ubiquitination in Tumorigenesis.Frontiers in cell and developmental biology · 2022Review
- Regulation of the endocytosis and prion-chaperoning machineries by yeast E3 ubiquitin ligase Rsp5 as revealed by orthogonal ubiquitin transfer.Cell chemical biology · 2021Article
- Article
- Regulation of Protein Post-Translational Modifications on Metabolism of Actinomycetes.Biomolecules · 2020Review
- Regulation of large and small G proteins by ubiquitination.The Journal of biological chemistry · 2019Review
- Regulation of glioma migration and invasion via modification of Rap2a activity by the ubiquitin ligase Nedd4-1.Oncology reports · 2017Article
- The ubiquitin-conjugating enzyme, Ubc1, indirectly regulates SNF1 kinase activity via Forkhead-dependent transcription.Microbial cell (Graz, Austria) · 2016Article
- Specific α-arrestins negatively regulate Saccharomyces cerevisiae pheromone response by down-modulating the G-protein-coupled receptor Ste2.Molecular and cellular biology · 2014Article
- The role of ubiquitin ligases in cardiac disease.Journal of molecular and cellular cardiology · 2014Review
- Guanine nucleotide-binding protein (Gα) endocytosis by a cascade of ubiquitin binding domain proteins is required for sustained morphogenesis and proper mating in yeast.The Journal of biological chemistry · 2014Article
- Regulation of yeast G protein signaling by the kinases that activate the AMPK homolog Snf1.Science signaling · 2013Article
- HIV-1 Nef impairs heterotrimeric G-protein signaling by targeting Gα(i2) for degradation through ubiquitination.The Journal of biological chemistry · 2012Article
- Proteasomal degradation of the metabotropic glutamate receptor 1α is mediated by Homer-3 via the proteasomal S8 ATPase: Signal transduction and synaptic transmission.Journal of neurochemistry · 2012Article
- Signal activation and inactivation by the Gα helical domain: a long-neglected partner in G protein signaling.Science signaling · 2012Review
- Yeast lipin 1 orthologue pah1p regulates vacuole homeostasis and membrane fusion.The Journal of biological chemistry · 2012Article
- Pheromone- and RSP5-dependent ubiquitination of the G protein beta subunit Ste4 in yeast.The Journal of biological chemistry · 2011Article
- Cell cycle-dependent phosphorylation and ubiquitination of a G protein alpha subunit.The Journal of biological chemistry · 2011Article
- Systematic analysis of essential genes reveals important regulators of G protein signaling.Molecular cell · 2010Article
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7 authors.
Funding
Abstract
Emerging evidence suggests that ubiquitination serves as a protein trafficking signal in addition to its well characterized role in promoting protein degradation. The yeast G protein alpha subunit Gpa1 represents a rare example of a protein that undergoes both mono- and poly-ubiquitination. Whereas mono-ubiquitinated Gpa1 is targeted to the vacuole, poly-ubiquitinated Gpa1 is directed instead to the proteasome. Here we investigate the structural requirements for mono- and poly-ubiquitination of Gpa1. We find that variants of Gpa1 engineered to be unstable are more likely to be poly-ubiquitinated and less likely to be mono-ubiquitinated. In addition, mutants that cannot be myristoylated are no longer mono-ubiquitinated but are still polyubiquitinated. Finally, we show that the ubiquitin ligase Rsp5 is necessary for Gpa1 mono-ubiquitination in vivo and that the purified enzyme is sufficient to catalyze Gpa1 mono-ubiquitination in vitro. Taken together, these data indicate that mono- and poly-ubiquitination have distinct enzyme and substrate recognition requirements; whereas poly-ubiquitination targets misfolded protein for degradation, a distinct ubiquitination apparatus targets the fully mature, fully myristoylated G protein for mono-ubiquitination and delivery to the vacuole.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.