ArticleBiomaterials2009
The influence of three-dimensional nanofibrous scaffolds on the osteogenic differentiation of embryonic stem cells.
Article in Biomaterials, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 39 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
39 citing papers in PubMed, 126 citations in OpenAlex.
- Synthetic Periodontal Guided Tissue Regeneration Membrane with Self-Assembling Biphasic Structure and Temperature-Sensitive Shape Maintenance.Advanced healthcare materials · 2025Article
- Release kinetics of the model protein FITC-BSA from different polymer-coated bovine bone substitutes.Head & face medicine · 2019Article
- Material and regenerative properties of an osteon-mimetic cortical bone-like scaffold.Regenerative biomaterials · 2019Article
- Tailoring weight ratio of PCL/PLA in electrospun three-dimensional nanofibrous scaffolds and the effect on osteogenic differentiation of stem cells.Colloids and surfaces. B, Biointerfaces · 2018Article
- Article
- Simvastatin and nanofibrous poly(l-lactic acid) scaffolds to promote the odontogenic potential of dental pulp cells in an inflammatory environment.Acta biomaterialia · 2018Article
- Expansion of mesenchymal stem cells on electrospun scaffolds maintains stemness, mechano-responsivity, and differentiation potential.Journal of orthopaedic research : official publication of the Orthopaedic Research Society · 2018Article
- Modeling, validation and verification of three-dimensional cell-scaffold contacts from terabyte-sized images.BMC bioinformatics · 2017Article
- A Bioinformatics 3D Cellular Morphotyping Strategy for Assessing Biomaterial Scaffold Niches.ACS biomaterials science & engineering · 2017Article
- Nanofiber scaffolds influence organelle structure and function in bone marrow stromal cells.Journal of biomedical materials research. Part B, Applied biomaterials · 2017Article
- Three dimensional electrospun PCL/PLA blend nanofibrous scaffolds with significantly improved stem cells osteogenic differentiation and cranial bone formation.Biomaterials · 2017Article
- Local pulsatile PTH delivery regenerates bone defects via enhanced bone remodeling in a cell-free scaffold.Biomaterials · 2017Article
- Combinatorial Method/High Throughput Strategies for Hydrogel Optimization in Tissue Engineering Applications.Gels (Basel, Switzerland) · 2016Review
- Nanostructured injectable cell microcarriers for tissue regeneration.Nanomedicine (London, England) · 2016Review
- Dentin regeneration by stem cells of apical papilla on injectable nanofibrous microspheres and stimulated by controlled BMP-2 release.Acta biomaterialia · 2016Article
- Cell-free 3D scaffold with two-stage delivery of miRNA-26a to regenerate critical-sized bone defects.Nature communications · 2016Article
- Structural properties of scaffolds: Crucial parameters towards stem cells differentiation.World journal of stem cells · 2015Review
- Hierarchical polymeric scaffolds support the growth of MC3T3-E1 cells.Journal of materials science. Materials in medicine · 2015Article
- Influence of discrete and continuous culture conditions on human mesenchymal stem cell lineage choice in RGD concentration gradient hydrogels.Biomacromolecules · 2013Article
- Maximizing phenotype constraint and extracellular matrix production in primary human chondrocytes using arginine-glycine-aspartate concentration gradient hydrogels.Acta biomaterialia · 2013Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
Embryonic stem cells represent a potentially unlimited cell source for tissue engineering applications. However, in order to be used for such applications, embryonic stem cells' differentiation must be controlled to only the desired lineages. In this study, we examine the effects of nanofibrous architecture and biochemical cues on the osteogenic differentiation of embryonic stem cells compared to the more traditional architecture without the nanofibrous features in two dimensions (thin matrix or flat films) and three dimensions (scaffolds) in vitro. After three weeks of culture the nanofibrous thin matrices were capable of supporting mRNA expression of osteogenic differentiation markers in embryonic stem cells without osteogenic supplements, while solid films required osteogenic supplements and growth factors to achieve mRNA expression of osteogenic differentiation markers. Nanofibrous scaffolds substantially enhanced mRNA expression of osteogenic differentiation markers compared to solid-walled scaffolds, nanofibrous thin matrices or solid films. After 4 weeks of culture, nanofibrous scaffolds were found to contain 3 times more calcium and stronger osteocalcin stain throughout the scaffolds than the solid-walled scaffolds. Overall, the nanofibrous architecture enhanced the osteogenic differentiation and mineralization of embryonic stem cells compared to the solid-walled architecture in both two and three-dimensional cultures.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.