ArticleJournal of cell science2009
Mechanically induced osteogenic differentiation--the role of RhoA, ROCKII and cytoskeletal dynamics.
Article in Journal of cell science, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 166 papers, 1 of them a synthesis that pooled it.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
166 citing papers in PubMed, 1 synthesis or guideline pooled it, 395 citations in OpenAlex.
- A multiethnic whole genome sequencing study to identify novel loci for bone mineral density.Human molecular genetics · 2022Pooled it
- Mechanical‑immune feedback regulation in distraction osteogenesis: From mechanotransduction to immune microenvironment remodeling (Review).Molecular medicine reports · 2026Review
- Biological mechanisms underlying the effects of low-magnitude mechanical stress in osteoporosis (Review).Experimental and therapeutic medicine · 2026Review
- SiOAdvanced healthcare materials · 2026Article
- Review
- Magnesium ions facilitate osteogenic differentiation and intervertebral fusionJournal of orthopaedic translation · 2026Article
- MicroRNA-mediated mechanotransduction and chondrocyte differentiation in mesenchymal stem cells.Animal cells and systems · 2026Review
- Mechanotransduction and cell fate: from molecular sensors to multicellular self-organization.Frontiers in cell and developmental biology · 2026Review
- Transcriptomic analysis reveals liver lncRNA-mRNA regulatory networks mediating Nicol1-induced growth and reproduction in golden pompano (Trachinotus ovatus).BMC genomics · 2025Article
- Bionic Nanostructures Create Mechanical Signals to Mediate the Composite Structural Bone Regeneration Through Multi-System Regulation.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2025Review
- Regulation of adipogenesis by nucleotides.Biochemical Society transactions · 2025Review
- Hydrogels with reversible stiffness modulation: new materials for studying dynamic mechanical cues that regulate cell behavior.Frontiers in bioengineering and biotechnology · 2025Review
- Inflammation and mechanical force-induced bone remodeling.Periodontology 2000 · 2024Review
- Effect of Structure on Osteogenesis of Bone Scaffold: Simulation Analysis Based on Mechanobiology and Animal Experiment Verification.Bioengineering (Basel, Switzerland) · 2024Article
- Advances in Shear Stress Stimulation of Stem Cells: A Review of the Last Three Decades.Biomedicines · 2024Review
- Integrated evaluation of biomechanical and biological properties of the biomimetic structural bone scaffold: Biomechanics, simulation analysis, and osteogenesis.Materials today. Bio · 2024Article
- Biophysics of ACL Injuries.Orthopedic reviews · 2024Article
- InDel and CNV within theAnimals : an open access journal from MDPI · 2023Article
- Assembling the Puzzle Pieces. Insights for in Vitro Bone Remodeling.Stem cell reviews and reports · 2023Review
- Mechanobiology-informed biomaterial and tissue engineering strategies for influencing skeletal stem and progenitor cell fate.Frontiers in physiology · 2023Review
106 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors at 3 institutions in 1 country.
Funding
Abstract
Many biochemical factors regulating progenitor cell differentiation have been examined in detail; however, the role of the local mechanical environment on stem cell fate has only recently been investigated. In this study, we examined whether oscillatory fluid flow, an exogenous mechanical signal within bone, regulates osteogenic, adipogenic or chondrogenic differentiation of C3H10T1/2 murine mesenchymal stem cells by measuring Runx2, PPARgamma and SOX9 gene expression, respectively. Furthermore, we hypothesized that the small GTPase RhoA and isometric tension within the actin cytoskeleton are essential in flow-induced differentiation. We found that oscillatory fluid flow induces the upregulation of Runx2, Sox9 and PPARgamma, indicating that it has the potential to regulate transcription factors involved in multiple unique lineage pathways. Furthermore, we demonstrate that the small GTPase RhoA and its effector protein ROCKII regulate fluid-flow-induced osteogenic differentiation. Additionally, activated RhoA and fluid flow have an additive effect on Runx2 expression. Finally, we show RhoA activation and actin tension are negative regulators of both adipogenic and chondrogenic differentiation. However, an intact, dynamic actin cytoskeleton under tension is necessary for flow-induced gene expression.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.