ArticleCalcified tissue international2009
Ablation of cathepsin k activity in the young mouse causes hypermineralization of long bone and growth plates.
Article in Calcified tissue international, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
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Who cites it
11 citing papers in PubMed, 30 citations in OpenAlex.
- Fumitremorgin C Attenuates Osteoclast Formation and FunctionFrontiers in pharmacology · 2020Article
- Effectiveness of elcatonin for alleviating pain and inhibiting bone resorption in patients with osteoporotic vertebral fractures.Journal of bone and mineral metabolism · 2017Article
- Cathepsin K Inhibitors for Osteoporosis: Biology, Potential Clinical Utility, and Lessons Learned.Endocrine reviews · 2017Review
- Human bone marrow-derived mesenchymal stem cells display enhanced clonogenicity but impaired differentiation with hypoxic preconditioning.Stem cells translational medicine · 2014Article
- ADAM17 controls endochondral ossification by regulating terminal differentiation of chondrocytes.Molecular and cellular biology · 2013Article
- Fourier transform infrared spectroscopic imaging parameters describing acid phosphate substitution in biologic hydroxyapatite.Calcified tissue international · 2013Article
- Article
- Proteases involved in cartilage matrix degradation in osteoarthritis.Biochimica et biophysica acta · 2012Review
- Impaired fracture healing in macrophage migration inhibitory factor-deficient mice.Osteoporosis international : a journal established as result of cooperation between the European Foundation for Osteoporosis and the National Osteoporosis Foundation of the USA · 2011Article
- Potential role of cathepsin K in the pathophysiology of mucopolysaccharidoses.Journal of pediatric rehabilitation medicine · 2010Article
- Differentiation and mineralization of murine mesenchymal C3H10T1/2 cells in micromass culture.Differentiation; research in biological diversityArticle
Corrections and comments
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Authors and funding
7 authors at 3 institutions in 1 country.
Funding
Abstract
Cathepsin K deficiency in humans causes pycnodysostosis, which is characterized by dwarfism and osteosclerosis. Earlier studies of 10-week-old male cathepsin K-deficient (knockout, KO) mice showed their bones were mechanically more brittle, while histomorphometry showed that both osteoclasts and osteoblasts had impaired activity relative to the wild type (WT). Here, we report detailed mineral and matrix analyses of the tibia of these animals based on Fourier transform infrared microspectroscopy and imaging. At 10 weeks, there was significant hypercalcification of the calcified cartilage and cortices in the KO. Carbonate content was elevated in the KO calcified cartilage as well as cortical and cancellous bone areas. These data suggest that cathepsin K does not affect mineral deposition but has a significant effect on mineralized tissue remodeling. Since growth plate abnormalities were extensive despite reported low levels of cathepsin K expression in the calcified cartilage, we used a differentiating chick limb-bud mesenchymal cell system that mimics endochondral ossification but does not contain osteoclasts, to show that cathepsin K inhibition during initial stages of mineral deposition retards the mineralization process while general inhibition of cathepsins can increase mineralization. These data suggest that the hypercalcification of the cathepsin K-deficient growth plate is due to persistence of calcified cartilage and point to a role of cathepsin K in bone tissue development as well as skeletal remodeling.
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