ArticlePloS one2009
Split-cre complementation indicates coincident activity of different genes in vivo.
Article in PloS one, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 80 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
80 citing papers in PubMed, 165 citations in OpenAlex.
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- Cell Fate Determination and Lineage Tracing: Technological Evolution and Multidimensional Applications.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2025Review
- Cross-Analysis of Single-Cell Transcriptomic Datasets Reveals Conserved Neurogenic Gene Signatures and New Insights Into Neural Stem Cell Aging.Aging cell · 2025Article
- Dual recombinase-mediated intersectional genetics defines the functional heterogeneity of neural stem cells in adult hippocampus.Molecular psychiatry · 2025Article
- IP3R2-Mediated Astrocytic CaCells · 2025Article
- HDL-bound S1P affects the subventricular niche and early neuropathological features of Alzheimer's disease.Nature communications · 2025Article
- Tackling Tissue Macrophage Heterogeneity by SplitCre Transgenesis.Methods in molecular biology (Clifton, N.J.) · 2024Article
- Established and emerging techniques for the study of microglia: visualization, depletion, and fate mapping.Frontiers in cellular neuroscience · 2024Review
- Visualization, Fate Mapping, Ablation, and Mutagenesis of Microglia in the Mouse Brain.Advances in neurobiology · 2024Article
- Design principles for synthetic control systems to engineer plants.Plant cell reports · 2023Review
- Commentary on: Bai, X., Zhao, N., Koupourtidou, C., Fang, L.-P., Schwarz, V., Caudal, L.C., Zhao, R., Hirrlinger, J., Walz, W., Bian, S., Huang, W., Ninkovic, J., Kirchhoff, F., Scheller, A. "In the mouse cortex, oligodendrocytes regain a plastic capacity, transforming into astrocytes after acute injury".Pflugers Archiv : European journal of physiology · 2023Article
- Understanding the Role of the Glial Scar through the Depletion of Glial Cells after Spinal Cord Injury.Cells · 2023Review
- Targeting osteocytes vs osteoblasts.Bone · 2023Review
- Review
- Cre-recombinase systems for induction of neuron-specific knockout models: a guide for biomedical researchers.Neural regeneration research · 2023Review
- Article
- Dentate gyrus astrocytes exhibit layer-specific molecular, morphological and physiological features.Nature neuroscience · 2022Article
- Construction of Attenuated Strains for Red-Spotted Grouper Nervous Necrosis Virus (RGNNV) via Reverse Genetic System.Viruses · 2022Article
- Article
20 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
11 authors at 5 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Cre/LoxP recombination is the gold standard for conditional gene regulation in mice in vivo. However, promoters driving the expression of Cre recombinase are often active in a wide range of cell types and therefore unsuited to target more specific subsets of cells. To overcome this limitation, we designed inactive "split-Cre" fragments that regain Cre activity when overlapping co-expression is controlled by two different promoters. Using transgenic mice and virus-mediated expression of split-Cre, we show that efficient reporter gene activation is achieved in vivo. In the brain of transgenic mice, we genetically defined a subgroup of glial progenitor cells in which the Plp1- and the Gfap-promoter are simultaneously active, giving rise to both astrocytes and NG2-positive glia. Similarly, a subset of interneurons was labelled after viral transfection using Gad67- and Cck1 promoters to express split-Cre. Thus, split-Cre mediated genomic recombination constitutes a powerful spatial and temporal coincidence detector for in vivo targeting.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.