ArticleGenetics2009
Tools for fungal proteomics: multifunctional neurospora vectors for gene replacement, protein expression and protein purification.
Article in Genetics, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 74 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
74 citing papers in PubMed, 152 citations in OpenAlex.
- Rhythmicity of TOR (Target of Rapamycin) Activity Supports Circadian Function inJournal of biological rhythms · 2026Article
- Circadian clock control of ribosome composition promotes rhythmic translation and termination fidelity.Cell reports · 2025Article
- The C-terminal domain of SEC-10 is fundamental for exocyst function, apical organization, and cell morphogenesis inMolecular biology of the cell · 2025Article
- Organelle activity organized by the endoplasmic reticulum-mitochondria encounter structure -ERMES- is essential forMicrobial cell (Graz, Austria) · 2025Article
- A constitutive heterochromatic region shapes genome organization and impacts gene expression in Neurospora crassa.BMC genomics · 2024Article
- Convergent evolution links molybdenum insertase domains with organism-specific sequences.Communications biology · 2024Article
- A Constitutive Heterochromatic Region Shapes Genome Organization and Impacts Gene Expression inbioRxiv : the preprint server for biology · 2024Article
- Rhythmidia: A modern tool for circadian period analysis of filamentous fungi.PLoS computational biology · 2024Article
- Unveiling a classical mutant in the context of the GH3 β-glucosidase family in Neurospora crassa.AMB Express · 2024Article
- CSE-8, a filamentous fungus-specific Shr3-like chaperone, facilitates endoplasmic reticulum exit of chitin synthase CHS-3 (class I) inFrontiers in fungal biology · 2024Article
- Establishment, optimization, and application of genetic technology inFrontiers in microbiology · 2023Review
- Article
- Light regulates the degradation of the regulatory protein VE-1 in the fungus Neurospora crassa.BMC biology · 2022Article
- TheFrontiers in microbiology · 2022Article
- Phenotype to genotype inCurrent research in microbial sciences · 2022Article
- Article
- Shared Components of the FRQ-Less Oscillator and TOR Pathway Maintain Rhythmicity inJournal of biological rhythms · 2021Article
- Chromatin accessibility profiling in Neurospora crassa reveals molecular features associated with accessible and inaccessible chromatin.BMC genomics · 2021Article
- A global search for novel transcription factors impacting the Neurospora crassa circadian clock.G3 (Bethesda, Md.) · 2021Article
- Golden Gate vectors for efficient gene fusion and gene deletion in diverse filamentous fungi.Current genetics · 2021Article
14 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
The completion of genome-sequencing projects for a number of fungi set the stage for detailed investigations of proteins. We report the generation of versatile expression vectors for detection and isolation of proteins and protein complexes in the filamentous fungus Neurospora crassa. The vectors, which can be adapted for other fungi, contain C- or N-terminal FLAG, HA, Myc, GFP, or HAT-FLAG epitope tags with a flexible poly-glycine linker and include sequences for targeting to the his-3 locus in Neurospora. To introduce mutations at native loci, we also made a series of knock-in vectors containing epitope tags followed by the selectable marker hph (resulting in hygromycin resistance) flanked by two loxP sites. We adapted the Cre/loxP system for Neurospora, allowing the selectable marker hph to be excised by introduction of Cre recombinase into a strain containing a knock-in cassette. Additionally, a protein purification method was developed on the basis of the HAT-FLAG tandem affinity tag system, which was used to purify HETEROCHROMATIN PROTEIN 1 (HP1) and associated proteins from Neurospora. As expected on the basis of yeast two-hybrid and co-immunoprecipitation (Co-IP) experiments, the Neurospora DNA methyltransferase DIM-2 was found in a complex with HP1. Features of the new vectors allowed for verification of an interaction between HP1 and DIM-2 in vivo by Co-IP assays on proteins expressed either from their native loci or from the his-3 locus.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.