ArticleGenetics2009
Expression of I-CreI endonuclease generates deletions within the rDNA of Drosophila.
Article in Genetics, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 31 papers.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
31 citing papers in PubMed, 47 citations in OpenAlex.
- ThemicroPublication biology · 2025Article
- RNA polymerase II-mediated rDNA transcription mediates rDNA copy number expansion in Drosophila.PLoS genetics · 2024Article
- rDNA magnification is a unique feature of germline stem cells.Proceedings of the National Academy of Sciences of the United States of America · 2023Article
- Intragenomic rDNA variation - the product of concerted evolution, mutation, or something in between?Heredity · 2023Review
- Y chromosome toxicity does not contribute to sex-specific differences in longevity.Nature ecology & evolution · 2023Article
- The retrotransposon R2 maintainsProceedings of the National Academy of Sciences of the United States of America · 2023Article
- Substantial rDNA copy number reductions alter timing of development and produce variable tissue-specific phenotypes in C. elegans.Genetics · 2023Article
- Under the magnifying glass: The ups and downs of rDNA copy number.Seminars in cell & developmental biology · 2023Review
- Varying strength of selection contributes to the intragenomic diversity of rRNA genes.Nature communications · 2022Article
- Characterization of Cerebellum-Specific Ribosomal DNA Epigenetic Modifications in Alzheimer's Disease: Should the Cerebellum Serve as a Control Tissue After All?Molecular neurobiology · 2020Article
- The processivity factor Pol32 mediates nuclear localization of DNA polymerase delta and prevents chromosomal fragile site formation in Drosophila development.PLoS genetics · 2019Article
- The peculiar genetics of the ribosomal DNA blurs the boundaries of transgenerational epigenetic inheritance.Chromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology · 2019Review
- Micronuclei Formation Is Prevented by Aurora B-Mediated Exclusion of HP1a from Late-Segregating Chromatin inGenetics · 2018Article
- The epigenetic regulator SIRT7 guards against mammalian cellular senescence induced by ribosomal DNA instability.The Journal of biological chemistry · 2018Article
- Article
- The Nucleolus: In Genome Maintenance and Repair.International journal of molecular sciences · 2017Review
- Aurora B-mediated localized delays in nuclear envelope formation facilitate inclusion of late-segregating chromosome fragments.Molecular biology of the cell · 2015Article
- Integration, Regulation, and Long-Term Stability of R2 Retrotransposons.Microbiology spectrum · 2015Review
- Transgenerational inheritance of diet-induced genome rearrangements in Drosophila.PLoS genetics · 2015Article
- Neurodegeneration-associated instability of ribosomal DNA.Biochimica et biophysica acta · 2014Article
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
The rDNA arrays in Drosophila contain the cis-acting nucleolus organizer regions responsible for forming the nucleolus and the genes for the 28S, 18S, and 5.8S/2S RNA components of the ribosomes and so serve a central role in protein synthesis. Mutations or alterations that affect the nucleolus organizer region have pleiotropic effects on genome regulation and development and may play a role in genomewide phenomena such as aging and cancer. We demonstrate a method to create an allelic series of graded deletions in the Drosophila Y-linked rDNA of otherwise isogenic chromosomes, quantify the size of the deletions using real-time PCR, and monitor magnification of the rDNA arrays as their functions are restored. We use this series to define the thresholds of Y-linked rDNA required for sufficient protein translation, as well as establish the rate of Y-linked rDNA magnification in Drosophila. Finally, we show that I-CreI expression can revert rDNA deletion phenotypes, suggesting that double-strand breaks are sufficient to induce rDNA magnification.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.