ArticleProceedings of the National Academy of Sciences of the United States of America2009
Yeast telomere capping protein Stn1 overrides DNA replication control through the S phase checkpoint.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
18 citing papers in PubMed, 23 citations in OpenAlex.
- Conditional deletion of human STN1 leads to telomere dysfunction, genome instability and proliferation defects.Journal of cell science · 2026Article
- Yeast Stn1 promotes MCM to circumvent Rad53 control of the S phase checkpoint.Current genetics · 2022Article
- The yeast Dbf4 ZnCurrent genetics · 2022Article
- Article
- Overlapping open reading frames strongly reduce human and yeast STN1 gene expression and affect telomere function.PLoS genetics · 2018Article
- Emerging roles of CST in maintaining genome stability and human disease.Frontiers in bioscience (Landmark edition) · 2018Review
- Progress in Human and Tetrahymena Telomerase Structure Determination.Annual review of biophysics · 2017Review
- STN1 OB Fold Mutation Alters DNA Binding and Affects Selective Aspects of CST Function.PLoS genetics · 2016Article
- The CDC13-STN1-TEN1 complex stimulates Pol α activity by promoting RNA priming and primase-to-polymerase switch.Nature communications · 2014Article
- Role of STN1 and DNA polymerase α in telomere stability and genome-wide replication in Arabidopsis.PLoS genetics · 2014Article
- Interplay between nonsense-mediated mRNA decay and DNA damage response pathways reveals that Stn1 and Ten1 are the key CST telomere-cap components.Cell reports · 2014Article
- Cdc13 OB2 dimerization required for productive Stn1 binding and efficient telomere maintenance.Structure (London, England : 1993) · 2013Article
- The telomere capping complex CST has an unusual stoichiometry, makes multipartite interaction with G-Tails, and unfolds higher-order G-tail structures.PLoS genetics · 2013Article
- Human CST promotes telomere duplex replication and general replication restart after fork stalling.The EMBO journal · 2012Article
- Evolution of CST function in telomere maintenance.Cell cycle (Georgetown, Tex.) · 2010Review
- A kinase-independent role for the Rad3(ATR)-Rad26(ATRIP) complex in recruitment of Tel1(ATM) to telomeres in fission yeast.PLoS genetics · 2010Article
- Protection and replication of telomeres in fission yeast.Biochemistry and cell biology = Biochimie et biologie cellulaire · 2009Review
- Telomere length regulation: coupling DNA end processing to feedback regulation of telomerase.The EMBO journal · 2009Review
Corrections and comments
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Authors and funding
11 authors at 2 institutions in 1 country.
Funding
Abstract
Telomere integrity is maintained through end-protection proteins that block nuclease degradation and prevent telomeres from being recognized as DNA breaks. Although less well understood, end protection proteins may also play a role in facilitating telomere replication. Here, we show that overproduction (OP) of the yeast telomere capping protein Stn1 makes cells highly sensitive to the replication inhibitors hydroxyurea (HU) and methyl-methane sulfonate (MMS). Unexpectedly, this sensitivity corresponds with Stn1 OP blocking most, if not all, aspects of the S phase checkpoint. The checkpoint kinase Rad53 is phosphorylated with normal timing in Stn1 OP cells, indicating Stn1 does not interfere with signaling steps involved in activating the checkpoint. Part of the role of Stn1 in telomere integrity is mediated through the Pol12 subunit of DNA polymerase alpha (Pol alpha). We show that overproduced Stn1 generally associates with chromosomes in HU treated and untreated cells, and, remarkably, Stn1 chromosome binding and OP checkpoint defects are rescued in pol12 mutants. We propose Stn1 normally promotes Pol alpha activity at telomeres but can be recruited through Pol12 to nontelomeric sites when overproduced. During replication stress, the mislocalized Stn1 may inappropriately promote Pol alpha in a manner that interferes with Rad53 effector mechanisms controlling replication fork integrity.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.