ArticleThe Journal of cell biology2009
Translocation or just location? Pseudopodia affect fluorescent signals.
Article in The Journal of cell biology, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
10 citing papers in PubMed.
- Intraphagosomal Free CaInternational journal of molecular sciences · 2024Article
- Cell-based optimization and characterization of genetically encoded location-based biosensors for Cdc42 or Rac activity.Journal of cell science · 2023Article
- Localisation of Intracellular Signals and Responses during Phagocytosis.International journal of molecular sciences · 2023Review
- Visualizing endogenous Rho activity with an improved localization-based, genetically encoded biosensor.Journal of cell science · 2021Article
- The myotubularin MTMR4 regulates phagosomal phosphatidylinositol 3-phosphate turnover and phagocytosis.The Journal of biological chemistry · 2019Article
- Localizing the lipid products of PI3Kγ in neutrophils.Advances in biological regulation · 2016Article
- Fluorescent Protein Based FRET Pairs with Improved Dynamic Range for Fluorescence Lifetime Measurements.PloS one · 2015Article
- Ca²⁺ and calpain control membrane expansion during the rapid cell spreading of neutrophils.Journal of cell science · 2013Article
- Bradykinin-induced chemotaxis of human gliomas requires the activation of KCa3.1 and ClC-3.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2013Article
- A reporter of UV intensity delivered to the cytosol during photolytic uncaging.Biophysical journal · 2010Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors.
Funding
Abstract
The use of fluorescent probes is one of the most powerful techniques for gaining spatial and temporal knowledge of dynamic events within living cells. Localized increases in the signal from cytosolic fluorescent protein constructs, for example, are frequently used as evidence for translocation of proteins to specific sites within the cell. However, differences in optical and geometrical properties of cytoplasm can influence the recorded intensity of the probe signal. Pseudopodia are especially problematic because their cytoplasmic properties can cause abrupt increases in fluorescent signal of both GFP and fluorescein. Investigators should therefore be cautious when interpreting fluorescence changes within a cell, as these can result from either translocation of the probe or changes in the optical properties of the milieu surrounding the probe.
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What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.