ArticlePlanta2009
The intergenic region of Arabidopsis thaliana cab1 and cab2 divergent genes functions as a bidirectional promoter.
Article in Planta, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 33 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
33 citing papers in PubMed, 52 citations in OpenAlex.
- RUBY-BD: a highly efficient visual reporter system for assessing bidirectional promoter activity.Planta · 2026Article
- Synthetic Biology of Plants and Microbes for Agriculture, Environment, and Future Applications.Chemical reviews · 2026Review
- Identification and application of bioparts for plant synthetic biology.Molecules and cells · 2025Review
- Comprehensive comparative analysis and development of molecular markers for Lasianthus species based on complete chloroplast genome sequences.BMC plant biology · 2024Article
- Identification and functional characterization of bidirectional gene pairs and their intergenic regions in cotton.BMC plant biology · 2024Article
- Shedding light on AT1G29480 ofPlant direct · 2022Article
- Isolation and Functional Characterization of a Green-Tissue Promoter in Japonica Rice (Biology · 2022Article
- A promoter toolbox for tissue-specific expression supporting translational research in cassava (Frontiers in plant science · 2022Article
- A synthetic C4 shuttle via the β-hydroxyaspartate cycle in C3 plants.Proceedings of the National Academy of Sciences of the United States of America · 2021Article
- Spatial transcriptional signatures define margin morphogenesis along the proximal-distal and medio-lateral axes in tomato (Solanum lycopersicum) leaves.The Plant cell · 2021Article
- Comprehensive construction strategy of bidirectional green tissue-specific synthetic promoters.Plant biotechnology journal · 2020Article
- Article
- Bidirectional Promoter-Based CRISPR-Cas9 Systems for Plant Genome Editing.Frontiers in plant science · 2019Article
- Plant Defenses Against Pests Driven by a Bidirectional Promoter.Frontiers in plant science · 2019Article
- Structural and Functional Analysis of a Bidirectional Promoter fromInternational journal of molecular sciences · 2018Article
- Expression of the Intracellular COPT3-Mediated Cu Transport Is Temporally Regulated by the TCP16 Transcription Factor.Frontiers in plant science · 2018Article
- Expression of a novel bi-directional Brassica napus promoter in soybean.Transgenic research · 2017Article
- Analysis of Promoters of Arabidopsis thaliana Divergent Gene Pair SERAT3;2 and IDH-III Shows SERAT3;2 Promoter is Nested Within the IDH-III Promoter.Molecular biotechnology · 2017Article
- A phosphate starvation-driven bidirectional promoter as a potential tool for crop improvement and in vitro plant biotechnology.Plant biotechnology journal · 2017Article
- Protection of Human Colon Cells from Shiga Toxin by Plant-based Recombinant Secretory IgA.Scientific reports · 2017Article
Corrections and comments
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Authors and funding
4 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Genetic engineering plays a unique role in fundamental plant biology studies and in improving crop traits. These efforts often necessitate introduction and expression of multiple genes using promoters from a very limited repertoire. Current common practice of expressing multiple genes is the repeated use of the same or similar promoters. This practice causes more frequent transgene silencing due to a high degree of sequence homology and a greater chance of rearrangement among repeatedly used promoter sequences. Therefore, availability and use of natural bidirectional promoters to minimize gene silencing and achieve desirable expression pattern of transgenes is a critical issue in the field of plant genetic engineering. Here we describe the use of a single natural bidirectional promoter to drive the expression of two reporter genes in onion epidermal cells and in transgenic tobacco plants. We show that (1) the promoter drives the simultaneous expression of GUS and GFP reporter genes after transient expression and stable transformation, (2) the transcription is equally strong in both directions, (3) immediate upstream regions in each direction control transcription independently from each other, and (4) the reporter genes are expressed in leaves and stems but not in roots, as expected from the fact that the endogenous promoter controls the expression of two photosynthetic genes in Arabidopsis. Hence, use of bidirectional promoters in heterologous background provides a means to express multiple genes in transgenic plants and aids genetic engineering-based crop improvement.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.