ArticleFrontiers in neuroanatomy2009
Dual transneuronal tracing in the rat entorhinal-hippocampal circuit by intracerebral injection of recombinant rabies virus vectors.
Article in Frontiers in neuroanatomy, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 29 papers.
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Who cites it
29 citing papers in PubMed.
- Barcoded Rabies In Situ Connectomics for high-throughput reconstruction of neural circuits.Nature communications · 2026Article
- Development and application of a barcoded rabies viral tracing method for mapping brain-wide inputs to single neurons.Cell reports methods · 2025Article
- Dorsal-Caudal and Ventral Hippocampi Target Different Cell Populations in the Medial Frontal Cortex in Rodents.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2025Article
- Self-replicating vehicles based on negative strand RNA viruses.Cancer gene therapy · 2023Review
- Spinal premotor interneurons controlling antagonistic muscles are spatially intermingled.eLife · 2022Article
- Self-Replicating RNA Viruses for Vaccine Development against Infectious Diseases and Cancer.Vaccines · 2021Review
- Article
- Optimization of whole-brain rabies virus tracing technology for small cell populations.Scientific reports · 2021Article
- Viral Vectors for COVID-19 Vaccine Development.Viruses · 2021Review
- Self-Amplifying RNA Viruses as RNA Vaccines.International journal of molecular sciences · 2020Review
- Trans-synaptic Neural Circuit-Tracing with Neurotropic Viruses.Neuroscience bulletin · 2019Review
- A whole-brain map of long-range inputs to GABAergic interneurons in the mouse medial prefrontal cortex.Nature neuroscience · 2019Article
- Review
- Self-Replicating RNA Viruses for RNA Therapeutics.Molecules (Basel, Switzerland) · 2018Review
- Gene Expression Profiling with Cre-Conditional Pseudorabies Virus Reveals a Subset of Midbrain Neurons That Participate in Reward Circuitry.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2017Article
- The functional logic of corticostriatal connections.Brain structure & function · 2017Review
- Increased transgene expression level of rabies virus vector for transsynaptic tracing.PloS one · 2017Article
- Article
- Long-term Cre-mediated retrograde tagging of neurons using a novel recombinant pseudorabies virus.Frontiers in neuroanatomy · 2014Article
- Rabies virus glycoprotein variants display different patterns in rabies monosynaptic tracing.Frontiers in neuroanatomy · 2014Article
Corrections and comments
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Authors and funding
8 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Dual transneuronal tracing is a novel viral tracing methodology which employs two recombinant viruses, each expressing a different reporter protein. Peripheral injection of recombinant pseudorabies viruses has been used as a powerful method to define neurons that coordinate outputs to various peripheral targets of motor and autonomic systems. Here, we assessed the feasibility of recombinants of rabies virus (RV) vector for dual transneuronal tracing in the central nervous system. First, we examined whether two different RV-vectors can double label cells in vitro, and showed that efficient double labeling can be realized by infecting targeted cells with the two RV-vectors within a short time interval. The potential of dual transneuronal tracing was then examined in vivo in the entorhinal-hippocampal circuit, using the chain of projections from CA3 pyramidal cells to CA1 pyramidal cells and subsequently to entorhinal cortex. Six days after the injection of two RV-vectors into the left and right entorhinal cortex respectively, double-labeled neurons were observed in CA3 bilaterally. Some double-labeled neurons showed a Golgi-like labeling. Dual transneuronal tracing potentially provides a powerful and sensitive method to study issues such as the amount of convergence and divergence within and between circuits in the central nervous system. Using this sensitive technique, we established that single neurons in CA3 are connected to the entorhinal cortex bilaterally with only one synaptic relay.
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