ArticleNature neuroscience2009
A chloride conductance in VGLUT1 underlies maximal glutamate loading into synaptic vesicles.
Article in Nature neuroscience, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 79 papers.
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Who cites it
79 citing papers in PubMed, 152 citations in OpenAlex.
- Substrate recognition and allosteric regulation of synaptic vesicle glutamate transporter VGLUT2.Nature structural & molecular biology · 2025Article
- Allosteric modulation of proton binding confers Cl- activation and glutamate selectivity to vesicular glutamate transporters.PLoS computational biology · 2025Article
- Structures of native SV2A reveal the binding mode for tetanus neurotoxin and anti-epileptic racetams.Nature communications · 2025Article
- Synaptophysin accelerates synaptic vesicle fusion by expanding the membrane upon neurotransmitter loading.Science advances · 2025Article
- An endogenous cholinergic system controls electrical conduction in the heart.European heart journal · 2025Article
- Dopamine-Glutamate and Dopamine-GABA Co-release.Advances in neurobiology · 2025Review
- Article
- Rett and Rett-related disorders: Common mechanisms for shared symptoms?Experimental biology and medicine (Maywood, N.J.) · 2023Review
- Vesicular glutamate transporters are HNature communications · 2023Article
- Diversity of function and mechanism in a family of organic anion transporters.Current opinion in structural biology · 2022Review
- Whole Endosome Recording of Vesicular Neurotransmitter Transporter Currents.Methods in molecular biology (Clifton, N.J.) · 2022Article
- Allosteric Inhibition of a Vesicular Glutamate Transporter by an Isoform-Specific Antibody.Biochemistry · 2021Article
- Sinoatrial node pacemaker cells share dominant biological properties with glutamatergic neurons.Protein & cell · 2021Article
- Cellular Physiology and Pathophysiology of EAAT Anion Channels.Frontiers in cellular neuroscience · 2021Review
- Physiological Perspectives on Molecular Mechanisms and Regulation of Vesicular Glutamate Transport: Lessons From Calyx of Held Synapses.Frontiers in cellular neuroscience · 2021Review
- Glutamic Acid Transporters: Targets for Neuroprotective Therapies in Parkinson's Disease.Frontiers in neuroscience · 2021Review
- Neurodegeneration Upon Dysfunction of Endosomal/Lysosomal CLC Chloride Transporters.Frontiers in cell and developmental biology · 2021Review
- The mechanism and regulation of vesicular glutamate transport: Coordination with the synaptic vesicle cycle.Biochimica et biophysica acta. Biomembranes · 2020Review
- Molecular, Structural, Functional, and Pharmacological Sites for Vesicular Glutamate Transporter Regulation.Molecular neurobiology · 2020Review
- Article
19 more citing papers are in PubMed but not listed here.
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Authors and funding
4 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Uptake of glutamate into synaptic vesicles is mediated by vesicular glutamate transporters (VGLUTs). Although glutamate uptake has been shown to depend critically on Cl(-), the precise contribution of this ion to the transport process is unclear. We found that VGLUT1, and not ClC-3 as proposed previously, represents the major Cl(-) permeation pathway in synaptic vesicles. Using reconstituted VGLUT1, we found that the biphasic dependence of glutamate transport on extravesicular Cl(-) is a result of the permeation of this anion through VGLUT1 itself. Moreover, we observed that high luminal Cl(-) concentrations markedly enhanced loading of glutamate by facilitation of membrane potential-driven uptake and discovered a hitherto unrecognized transport mode of VGLUT1. Because a steep Cl(-) gradient across the synaptic vesicle membrane exists in endocytosed synaptic vesicles, our results imply that the transport velocity and the final glutamate content are highly influenced, if not determined, by the extracellular Cl(-) concentration.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.