ArticleInvestigative ophthalmology & visual science2009
Depression of intraocular pressure following inactivation of connexin43 in the nonpigmented epithelium of the ciliary body.
Article in Investigative ophthalmology & visual science, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed, 30 citations in OpenAlex.
- Uveitic glaucoma-like features in Yap conditional knockout mice.Cell death discovery · 2024Article
- Gap junctions and connexin hemichannels both contribute to the electrical properties of retinal pigment epithelium.The Journal of general physiology · 2022Article
- The Role of GJD2(Cx36) in Refractive Error Development.Investigative ophthalmology & visual science · 2022Review
- Step-by-step preparation of mouse eye sections for routine histology, immunofluorescence, and RNASTAR protocols · 2021Article
- Altered Iris Aquaporin Expression and Aqueous Humor Osmolality in Glaucoma.Investigative ophthalmology & visual science · 2021Article
- NOTCH Signaling Controls Ciliary Body Morphogenesis and Secretion by Directly Regulating Nectin Protein Expression.Cell reports · 2021Article
- Gene Therapy for Glaucoma by Ciliary Body Aquaporin 1 Disruption Using CRISPR-Cas9.Molecular therapy : the journal of the American Society of Gene Therapy · 2020Article
- Effects of Latanoprost and Bimatoprost on the Expression of Molecules Relevant to Ocular Inflow and Outflow Pathways.PloS one · 2016Article
- Roles and regulation of lens epithelial cell connexins.FEBS letters · 2014Review
- Connexins form functional hemichannels in porcine ciliary epithelium.Experimental eye research · 2014Article
- Cell autonomous requirement of connexin 43 for osteocyte survival: consequences for endocortical resorption and periosteal bone formation.Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research · 2012Article
- Permeation pathway of homomeric connexin 26 and connexin 30 channels investigated by molecular dynamics.Journal of biomolecular structure & dynamics · 2012Article
- Biological physics in México: Review and new challenges.Journal of biological physics · 2011Article
- The expression of Wnt2b in the optic cup lip requires a border between the pigmented and nonpigmented epithelium.Molecular vision · 2010Article
- Regulation of gap junction coupling in bovine ciliary epithelium.American journal of physiology. Cell physiology · 2010Article
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Authors and funding
6 authors at 3 institutions in 2 countries.
Funding
Abstract
purposeConditional inactivation of connexin43 (Cx43) in the pigmented epithelium of the mouse eye results in a reduction in aqueous humor production and complete loss of the vitreous chamber. It was proposed that gap junctions between pigmented and nonpigmented epithelia of the ciliary body are critical for the production of the aqueous humor. To form such junctions, Cx43 in the pigmented epithelium must interact with connexin(s) present in the adjacent cells of the nonpigmented epithelium. The importance of Cx43 expression in the nonpigmented epithelium for the establishment of gap junctions and the regulation of intraocular pressure was tested.
methodsTo inactivate Cx43 in the nonpigmented epithelium of the mouse eye, a mouse line was crossed with a floxed Cx43 locus (Cx43(flox/flox)) and a transgenic mouse line expressing cre recombinase under the control of the Pax6alpha promoter. General eye structure was evaluated by light microscopy, gap junctions were analyzed by electron microscopy, and intraocular pressure was directly assessed with micropipettes.
resultsIn Pax6alpha-cre/Cx43(flox/flox) mice, Cx43 was partially inactivated in the nonpigmented epithelium of the ciliary body and iris. Animals developed dilatations between the pigmented and nonpigmented epithelia and displayed a significant reduction in intraocular pressure. However, gap junctions between the ciliary epithelial layers were decreased but not eliminated.
conclusionsCx43 expression in the nonpigmented epithelium of the ciliary body contributes to the formation of gap junctions with the cells of the pigmented epithelium. These gap junctions play a critical role in maintaining the physical integrity of the ciliary body epithelium. Although the partial loss of Cx43 from the nonpigmented epithelium was correlated with a measurable drop in intraocular pressure, possible changes in Cx43 in the aqueous outflow pathway may provide an additional contribution to the observed phenotype.
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