ArticleBiophysical journal2009
Regulation of cell cytoskeleton and membrane mechanics by electric field: role of linker proteins.
Article in Biophysical journal, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 59 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
59 citing papers in PubMed, 174 citations in OpenAlex.
- Magnetic Fields in Cancer Therapy: Mechanistic Insights, Signaling Pathways, and Evidence from Clinical and In Vitro Studies.Pharmaceutics · 2026Review
- The Bionic Interface: Considering the Material Mediated Electrical Stimulation of Stem Cells.Advanced materials (Deerfield Beach, Fla.) · 2026Review
- At the Pointy End of Mechanobiology: AFM for Transient Biomechanical Analysis.Advanced healthcare materials · 2025Review
- Recent advancements in tissue dissociation techniques for cell manufacturing single-cell analysis and downstream processing.Stem cells translational medicine · 2025Review
- Open Challenges and Opportunities in Piezoelectricity for Tissue Regeneration.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2025Review
- Review
- Electrical Stimulation Therapy - Dedicated to the Perfect Plastic Repair.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2025Review
- Assessment of molecular modulation by multifrequency electromagnetic pulses to preferably eradicate tumorigenic cells.Scientific reports · 2024Article
- Review
- Engineering 3D Scaffold-Free Nanoparticle-Laden Stem Cell Constructs for Piezoelectric Enhancement of Human Neural Tissue Formation and Function.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2024Article
- Stimuli-responsive microcarriers and their application in tissue repair: A review of magnetic and electroactive microcarrier.Bioactive materials · 2024Review
- Conductive and alignment-optimized porous fiber conduits with electrical stimulation for peripheral nerve regeneration.Materials today. Bio · 2024Article
- Electroactive 4D Porous Scaffold Based on Conducting Polymer as a Responsive and DynamicACS applied materials & interfaces · 2024Article
- Electrical Stimulation of Human Mesenchymal Stem Cells on Conductive Substrates Promotes Neural Priming.Macromolecular bioscience · 2023Article
- Self-promoted electroactive biomimetic mineralized scaffolds for bacteria-infected bone regeneration.Nature communications · 2023Article
- Active learning model for extracting elastic modulus of cell on substrate.Biophysical journal · 2023Article
- Advances in Conductive Hydrogel for Spinal Cord Injury Repair and Regeneration.International journal of nanomedicine · 2023Review
- Electroconductivity, a regenerative engineering approach to reverse rotator cuff muscle degeneration.Regenerative biomaterials · 2023Review
- Automated cell-type classification combining dilated convolutional neural networks with label-free acoustic sensing.Scientific reports · 2022Article
- Quantitative Investigation of the Link between Actin Cytoskeleton Dynamics and Cellular Behavior.Micromachines · 2022Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
Cellular mechanics is known to play an important role in the cell homeostasis including proliferation, motility, and differentiation. Significant variation in the mechanical properties between different cell types suggests that control of the cell metabolism is feasible through manipulation of the cell mechanical parameters using external physical stimuli. We investigated the electrocoupling mechanisms of cellular biomechanics modulation by an electrical stimulation in two mechanically distinct cell types--human mesenchymal stem cells and osteoblasts. Application of a 2 V/cm direct current electric field resulted in approximately a twofold decrease in the cell elasticity and depleted intracellular ATP. Reduction in the ATP level led to inhibition of the linker proteins that are known to physically couple the cell membrane and cytoskeleton. The membrane separation from the cytoskeleton was confirmed by up to a twofold increase in the membrane tether length that was extracted from the cell membrane after an electrical stimulation. In comparison to human mesenchymal stem cells, the membrane-cytoskeleton attachment in osteoblasts was much stronger but, in response to the same electrical stimulation, the membrane detachment from the cytoskeleton was found to be more pronounced. The observed effects mediated by an electric field are cell type- and serum-dependent and can potentially be used for electrically assisted cell manipulation. An in-depth understanding and control of the mechanisms to regulate cell mechanics by external physical stimulus (e.g., electric field) may have great implications for stem cell-based tissue engineering and regenerative medicine.
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What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.