ArticleBiophysical journal2009
Detecting protein complexes in living cells from laser scanning confocal image sequences by the cross correlation raster image spectroscopy method.
Article in Biophysical journal, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 66 papers.
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Who cites it
66 citing papers in PubMed.
- Mapping Protein Diffusion from the Plasma Membrane to the Nucleus: Insights from Fluorescence Correlation Spectroscopy.Biomolecules · 2026Review
- Fluorescence Correlation Spectroscopy to Examine Protein-Lipid Interactions in Membranes.Methods in molecular biology (Clifton, N.J.) · 2026Article
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- Heterochromatin protein 1 alpha (HP1α) undergoes a monomer to dimer transition that opens and compacts live cell genome architecture.Nucleic acids research · 2024Article
- Hetero-pentamerization determines mobility and conductance of Glycine receptor α3 splice variants.Cellular and molecular life sciences : CMLS · 2022Article
- The microprotein Nrs1 rewires the G1/S transcriptional machinery during nitrogen limitation in budding yeast.PLoS biology · 2022Article
- Mandipropamid as a chemical inducer of proximity for in vivo applications.Nature chemical biology · 2022Article
- Measuring the Lateral Diffusion of Plasma Membrane Receptors Using Raster Image Correlation Spectroscopy.Methods in molecular biology (Clifton, N.J.) · 2022Article
- A dominant-negative SOX18 mutant disrupts multiple regulatory layers essential to transcription factor activity.Nucleic acids research · 2021Article
- Quantitative cell biology of tip growth in moss.Plant molecular biology · 2021Review
- Multicolor fluorescence fluctuation spectroscopy in living cells via spectral detection.eLife · 2021Article
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- Recent evolution of a TET-controlled and DPPA3/STELLA-driven pathway of passive DNA demethylation in mammals.Nature communications · 2020Article
- Spatiotemporal dynamics of 53BP1 dimer recruitment to a DNA double strand break.Nature communications · 2020Article
- Lateral diffusion of CD14 and TLR2 in macrophage plasma membrane assessed by raster image correlation spectroscopy and single particle tracking.Scientific reports · 2020Article
- Fluctuation Imaging of LRRK2 Reveals that the G2019S Mutation Alters Spatial and Membrane Dynamics.Molecules (Basel, Switzerland) · 2020Article
- Fluorescence microscopy data for quantitative mobility and interaction analysis of proteins in living cells.Data in brief · 2020Article
- Syncrip/hnRNP Q is required for activity-induced Msp300/Nesprin-1 expression and new synapse formation.The Journal of cell biology · 2020Article
- Nanoscale Distribution of Nuclear Sites by Super-Resolved Image Cross-Correlation Spectroscopy.Biophysical journal · 2019Article
6 more citing papers are in PubMed but not listed here.
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4 authors.
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Abstract
We describe a general method for detecting molecular complexes based on the analysis of single molecule fluorescence fluctuations from laser scanning confocal images. The method detects and quantifies complexes of two different fluorescent proteins noninvasively in living cells. Because in a raster scanned image successive pixels are measured at different times, the spatial correlation of the image contains information about dynamic processes occurring over a large time range, from the microseconds to seconds. The correlation of intensity fluctuations measured simultaneously in two channels detects protein complexes that carry two molecules of different colors. This information is obtained from the entire image. A map of the spatial distribution of protein complexes in the cell and their diffusion and/or binding properties can be constructed. Using this cross correlation raster image spectroscopy method, specific locations in the cell can be visualized where dynamics of binding and unbinding of fluorescent protein complexes occur. This fluctuation imaging method can be applied to commercial laser scanning microscopes thereby making it accessible to a large community of scientists.
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