ArticleBiophysical journal2009
Quantitative transcription factor binding kinetics at the single-molecule level.
Article in Biophysical journal, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 29 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
29 citing papers in PubMed, 57 citations in OpenAlex.
- Mechanisms for DNA Interplay in Eukaryotic Transcription Factors.Annual review of biophysics · 2025Review
- Assembly mechanism of the inflammasome sensor AIM2 revealed by single molecule analysis.Nature communications · 2023Article
- Multiplexed Single-Molecule Experiments Reveal Nucleosome Invasion Dynamics of the Cas9 Genome Editor.Journal of the American Chemical Society · 2021Article
- KERA: analysis tool for multi-process, multi-state single-molecule data.Nucleic acids research · 2021Article
- The relation between crosstalk and gene regulation form revisited.PLoS computational biology · 2020Article
- Dissecting the binding mechanisms of transcription factors to DNA using a statistical thermodynamics framework.Computational and structural biotechnology journal · 2020Article
- Force-Dependent Binding Constants.Biochemistry · 2019Review
- Homotypic cooperativity and collective binding are determinants of bHLH specificity and function.Proceedings of the National Academy of Sciences of the United States of America · 2019Article
- Single-molecule kinetic analysis of HP1-chromatin binding reveals a dynamic network of histone modification and DNA interactions.Nucleic acids research · 2017Article
- The role of repressor kinetics in relief of transcriptional interference between convergent promoters.Nucleic acids research · 2016Article
- What have single-molecule studies taught us about gene expression?Genes & development · 2016Review
- Quantifying the Assembly of Multicomponent Molecular Machines by Single-Molecule Total Internal Reflection Fluorescence Microscopy.Methods in enzymology · 2016Article
- A general mechanism for competitor-induced dissociation of molecular complexes.Nature communications · 2014Article
- Direct optical mapping of transcription factor binding sites on field-stretched λ-DNA in nanofluidic devices.Nucleic acids research · 2014Article
- Molecular crowding shapes gene expression in synthetic cellular nanosystems.Nature nanotechnology · 2013Article
- RNA polymerase approaches its promoter without long-range sliding along DNA.Proceedings of the National Academy of Sciences of the United States of America · 2013Article
- Cross talk and interference enhance information capacity of a signaling pathway.Biophysical journal · 2013Article
- Specific labeling of zinc finger proteins using noncanonical amino acids and copper-free click chemistry.Bioconjugate chemistry · 2012Article
- Stretching DNA to quantify nonspecific protein binding.Physical review. E, Statistical, nonlinear, and soft matter physics · 2012Article
- Origins of concentration dependence of waiting times for single-molecule fluorescence binding.The Journal of chemical physics · 2012Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors at 1 institution in 1 country.
Funding
Abstract
We investigated the binding interaction between the bacteriophage lambda-repressor CI and its target DNA using total internal reflection fluorescence microscopy. Large stepwise changes in the intensity of the red fluorescent protein fused to CI were observed as it associated with and dissociated from individually labeled single-molecule DNA targets. The stochastic association and dissociation were characterized by Poisson statistics. Dark and bright intervals were measured for thousands of individual events. The exponential distribution of the intervals allowed direct determination of the association and dissociation rate constants (k(a) and k(d), respectively). We resolved in detail how k(a) and k(d) varied as a function of three control parameters: the DNA length L, the CI dimer concentration, and the binding affinity. Our results show that although interactions with nonoperator DNA sequences are observable, CI binding to the operator site is not dependent on the length of flanking nonoperator DNA.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.