ArticleBMC cancer2009
The intraportal injection model: a practical animal model for hepatic metastases and tumor cell dissemination in human colon cancer.
Article in BMC cancer, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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16 citing papers in PubMed, 29 citations in OpenAlex.
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- The liver microenvironment orchestrates FGL1-mediated immune escape and progression of metastatic colorectal cancer.Nature communications · 2023Article
- The Provocative Roles of Platelets in Liver Disease and Cancer.Frontiers in oncology · 2021Review
- AAV-mediated gene transfer of DNase I in the liver of mice with colorectal cancer reduces liver metastasis and restores local innate and adaptive immune response.Molecular oncology · 2020Article
- Reactivating p53 and Inducing Tumor Apoptosis (RITA) Enhances the Response of RITA-Sensitive Colorectal Cancer Cells to Chemotherapeutic Agents 5-Fluorouracil and Oxaliplatin.Neoplasia (New York, N.Y.) · 2017Article
- A Portal Vein Injection Model to Study Liver Metastasis of Breast Cancer.Journal of visualized experiments : JoVE · 2016Article
- Prrx1 isoform switching regulates pancreatic cancer invasion and metastatic colonization.Genes & development · 2016Article
- Preclinical therapeutic response of residual metastatic disease is distinct from its primary tumor of origin.International journal of cancer · 2012Article
- Pregnane X receptor activation induces FGF19-dependent tumor aggressiveness in humans and mice.The Journal of clinical investigation · 2011Article
- In vivo imaging of pancreatic tumours and liver metastases using 7 Tesla MRI in a murine orthotopic pancreatic cancer model and a liver metastases model.BMC cancer · 2011Article
- Evaluation of immunological escape mechanisms in a mouse model of colorectal liver metastases.BMC cancer · 2010Article
- Evaluation of metastatic and angiogenic potentials of human colon carcinoma cells in chick embryo model systems.Clinical & experimental metastasis · 2009Article
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Corrections and comments
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Authors and funding
10 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundThe development of new therapeutic strategies for treatment of metastasized colorectal carcinoma requires biologically relevant and adequate animal models that generate both reproducible metastasis and the dissemination of tumor cells in the form of so-called minimal residual disease (MRD), an expression of the systemic character of neoplastic disease.
methodsWe injected immunoincompetent nude mice intraportally with different numbers (1 x 10(5), 1 x 10(6) and 5 x 10(6) cells) of the human colon carcinoma cell lines HT-29 and SW-620 and investigated by histological studies and CK-20 RT-PCR the occurrence of hematogenous metastases and the dissemination of human tumor cells in bone marrow.
resultsOnly the injection of 1 x 10(6) cells of each colon carcinoma cell line produced acceptable perioperative mortality with reproducible induction of hepatic metastases in up to 89% of all animals. The injection of 1 x 10(6) cells also generated tumor cell dissemination in the bone marrow in up to 63% of animals with hepatic metastases.
conclusionThe present intraportal injection model in immunoincompetent nude mice represents a biologically relevant and adequate animal model for the induction of both reproducible hepatic metastasis and tumor cell dissemination in the bone marrow as a sign of MRD.
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