ArticlePloS one2009
Interplay of Mre11 nuclease with Dna2 plus Sgs1 in Rad51-dependent recombinational repair.
Article in PloS one, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 48 papers.
What it found
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
48 citing papers in PubMed, 65 citations in OpenAlex.
- Nej1 interacts with Sae2 at DNA double-stranded breaks to inhibit DNA resection.The Journal of biological chemistry · 2022Article
- Biochemical and structural characterization of analogs of MRE11 breast cancer-associated mutant F237C.Scientific reports · 2021Article
- The human HELLS chromatin remodelling protein promotes end resection to facilitate homologous recombination and contributes to DSB repair within heterochromatin.Nucleic acids research · 2020Article
- Multiple roles of DNA2 nuclease/helicase in DNA metabolism, genome stability and human diseases.Nucleic acids research · 2020Review
- CDK and Mec1/Tel1-catalyzed phosphorylation of Sae2 regulate different responses to DNA damage.Nucleic acids research · 2019Article
- Novel Phenotypes and Cardiac Involvement Associated With DNA2 Genetic Variants.Frontiers in neurology · 2019Article
- Sae2 antagonizes Rad9 accumulation at DNA double-strand breaks to attenuate checkpoint signaling and facilitate end resection.Proceedings of the National Academy of Sciences of the United States of America · 2018Article
- Review
- Genetic Separation of Sae2 Nuclease Activity from Mre11 Nuclease Functions in Budding Yeast.Molecular and cellular biology · 2017Article
- The SWI/SNF ATP-dependent nucleosome remodeler promotes resection initiation at a DNA double-strand break in yeast.Nucleic acids research · 2017Article
- Genetic and biochemical evidences reveal novel insights into the mechanism underlyingJournal of biosciences · 2016Article
- Xrs2 Dependent and Independent Functions of the Mre11-Rad50 Complex.Molecular cell · 2016Article
- A Selective Small Molecule DNA2 Inhibitor for Sensitization of Human Cancer Cells to Chemotherapy.EBioMedicine · 2016Article
- Emerging critical roles of Fe-S clusters in DNA replication and repair.Biochimica et biophysica acta · 2015Review
- Yeast Pif1 accelerates annealing of complementary DNA strands.Biochemistry · 2014Article
- The Mre11/Rad50/Nbs1 complex: recent insights into catalytic activities and ATP-driven conformational changes.Experimental cell research · 2014Review
- End resection at double-strand breaks: mechanism and regulation.Cold Spring Harbor perspectives in biology · 2014Review
- Preventing over-resection by DNA2 helicase/nuclease suppresses repair defects in Fanconi anemia cells.Cell cycle (Georgetown, Tex.) · 2014Article
- PCNA promotes processive DNA end resection by Exo1.Nucleic acids research · 2013Article
- Dna2 is involved in CA strand resection and nascent lagging strand completion at native yeast telomeres.The Journal of biological chemistry · 2013Article
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
The Mre11/Rad50/Xrs2 complex initiates IR repair by binding to the end of a double-strand break, resulting in 5' to 3' exonuclease degradation creating a single-stranded 3' overhang competent for strand invasion into the unbroken chromosome. The nuclease(s) involved are not well understood. Mre11 encodes a nuclease, but it has 3' to 5', rather than 5' to 3' activity. Furthermore, mutations that inactivate only the nuclease activity of Mre11 but not its other repair functions, mre11-D56N and mre11-H125N, are resistant to IR. This suggests that another nuclease can catalyze 5' to 3' degradation. One candidate nuclease that has not been tested to date because it is encoded by an essential gene is the Dna2 helicase/nuclease. We recently reported the ability to suppress the lethality of a dna2Delta with a pif1Delta. The dna2Delta pif1Delta mutant is IR-resistant. We have determined that dna2Delta pif1Delta mre11-D56N and dna2Delta pif1Delta mre11-H125N strains are equally as sensitive to IR as mre11Delta strains, suggesting that in the absence of Dna2, Mre11 nuclease carries out repair. The dna2Delta pif1Delta mre11-D56N triple mutant is complemented by plasmids expressing Mre11, Dna2 or dna2K1080E, a mutant with defective helicase and functional nuclease, demonstrating that the nuclease of Dna2 compensates for the absence of Mre11 nuclease in IR repair, presumably in 5' to 3' degradation at DSB ends. We further show that sgs1Delta mre11-H125N, but not sgs1Delta, is very sensitive to IR, implicating the Sgs1 helicase in the Dna2-mediated pathway.
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What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.