ArticleProceedings of the National Academy of Sciences of the United States of America2009
The nature of the TRAP-Anti-TRAP complex.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
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Who cites it
25 citing papers in PubMed, 33 citations in OpenAlex.
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- PinMyMetal: a hybrid learning system to accurately model transition metal binding sites in macromolecules.Nature communications · 2025Article
- Structural basis of nearest-neighbor cooperativity in the ring-shaped gene regulatory protein TRAP from protein engineering and cryo-EM.Proceedings of the National Academy of Sciences of the United States of America · 2025Article
- Solution structure, dynamics and tetrahedral assembly of Anti-TRAP, a homo-trimeric triskelion-shaped regulator of tryptophan biosynthesis inJournal of structural biology: X · 2024Article
- Structural basis of nearest-neighbor cooperativity in the ring-shaped gene regulatory protein TRAP from protein engineering and cryo-EM.bioRxiv : the preprint server for biology · 2024Article
- PinMyMetal: A hybrid learning system to accurately model metal binding sites in macromolecules.Research square · 2024Article
- Shape-Morphing of an Artificial Protein Cage with Unusual Geometry Induced by a Single Amino Acid Change.ACS nanoscience Au · 2022Article
- Thermodynamic coupling between neighboring binding sites in homo-oligomeric ligand sensing proteins from mass resolved ligand-dependent population distributions.Protein science : a publication of the Protein Society · 2022Article
- Artificial Protein Cage with Unusual Geometry and Regularly Embedded Gold Nanoparticles.Nano letters · 2022Article
- Artificial Protein Cage Delivers Active Protein Cargos to the Cell Interior.Biomacromolecules · 2021Article
- Biophysical reviews 'meet the editor series'-Jeremy R. H. Tame.Biophysical reviews · 2021Article
- Analytical ultracentrifugation in structural biology.Biophysical reviews · 2018Review
- Resurrecting the Dead (Molecules).Computational and structural biotechnology journal · 2017Review
- Computational design of a self-assembling symmetrical β-propeller protein.Proceedings of the National Academy of Sciences of the United States of America · 2014Article
- Modulating TRAP-mediated transcription termination by AT during transcription of the leader region of the Bacillus subtilis trp operon.Nucleic acids research · 2014Article
- Gene regulation by substoichiometric heterocomplex formation of undecameric TRAP and trimeric anti-TRAP.Proceedings of the National Academy of Sciences of the United States of America · 2014Article
- Homotropic cooperativity from the activation pathway of the allosteric ligand-responsive regulatory trp RNA-binding attenuation protein.Biochemistry · 2013Article
- Mechanisms of allosteric gene regulation by NMR quantification of microsecond-millisecond protein dynamics.Journal of molecular biology · 2012Article
- Influence of structural symmetry on protein dynamics.PloS one · 2012Article
- Crystal structure of unliganded TRAP: implications for dynamic allostery.The Biochemical journal · 2011Article
Corrections and comments
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Authors and funding
7 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Tryptophan biosynthesis is subject to exquisite control in species of Bacillus and has become one of the best-studied model systems in gene regulation. The protein TRAP (trp RNA-binding attenuation protein) predominantly forms a ring-shaped 11-mer, which binds cognate RNA in the presence of tryptophan to suppress expression of the trp operon. TRAP is itself regulated by the protein Anti-TRAP, which binds to TRAP and prevents RNA binding. To date, the nature of this interaction has proved elusive. Here, we describe mass spectrometry and analytical centrifugation studies of the complex, and 2 crystal structures of the TRAP-Anti-TRAP complex. These crystal structures, both refined to 3.2-A resolution, show that Anti-TRAP binds to TRAP as a trimer, sterically blocking RNA binding. Mass spectrometry shows that 11-mer TRAP may bind up to 5 AT trimers, and an artificial 12-mer TRAP may bind 6. Both forms of TRAP make the same interactions with Anti-TRAP. Crystallization of wild-type TRAP with Anti-TRAP selectively pulls the 12-mer TRAP form out of solution, so the crystal structure of wild-type TRAP-Anti-TRAP complex reflects a minor species from a mixed population.
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