ArticleProceedings of the National Academy of Sciences of the United States of America2009
Image-guided, noninvasive, spatiotemporal control of gene expression.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 44 papers.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
44 citing papers in PubMed, 98 citations in OpenAlex.
- Ultrasound priming gated by solid tumor hallmarks to guide CAR-T therapy.Science advances · 2026Article
- Tumour priming by ultrasound mechanogenetics for CAR T therapy.Nature materials · 2026Article
- An integrated ultrasound-guided focused ultrasound system enables spatiotemporal control of thermal gene activation in engineered immune cells.Theranostics · 2026Article
- Thermally Controlled State Switches for Engineered Macrophages.ACS synthetic biology · 2025Article
- Decoding paradoxical BOLD responses to transcranial ultrasound stimulation with concurrent optoacoustic magnetic resonance imaging.Science advances · 2025Article
- Overview of Therapeutic Ultrasound Applications and Safety Considerations: 2024 Update.Journal of ultrasound in medicine : official journal of the American Institute of Ultrasound in Medicine · 2025Review
- Advanced Strategies for Ultrasound Control and Applications in Sonogenetics and Gas Vesicle-Based Technologies: A Review.International journal of nanomedicine · 2025Review
- Ultrasound Control of Genomic Regulatory Toolboxes for Cancer Immunotherapy.Nature communications · 2024Article
- Significant Advancements and Evolutions in Chimeric Antigen Receptor Design.International journal of molecular sciences · 2024Review
- Advanced gene nanocarriers/scaffolds in nonviral-mediated delivery system for tissue regeneration and repair.Journal of nanobiotechnology · 2024Review
- Analyzing Gene Expression After Administration of Low-Intensity Therapeutic Ultrasound in Human Islet Cells.Journal of ultrasound in medicine : official journal of the American Institute of Ultrasound in Medicine · 2024Article
- The principles and promising future of sonogenetics for precision medicine.Theranostics · 2024Review
- Real-time automatic temperature regulation during in vivo MRI-guided laser-induced thermotherapy (MR-LITT).Scientific reports · 2023Article
- Iron-Based Magnetic Nanosystems for Diagnostic Imaging and Drug Delivery: Towards Transformative Biomedical Applications.Pharmaceutics · 2022Review
- Magnetic cryogels as a shape-selective and customizable platform for hyperthermia-mediated drug delivery.Scientific reports · 2022Article
- Engineering CAR T cells for enhanced efficacy and safety.APL bioengineering · 2022Review
- Synergies between therapeutic ultrasound, gene therapy and immunotherapy in cancer treatment.Advanced drug delivery reviews · 2021Review
- Control of the activity of CAR-T cells within tumours via focused ultrasound.Nature biomedical engineering · 2021Article
- Review
- Heat-Triggered Remote Control of CRISPR-dCas9 for Tunable Transcriptional Modulation.ACS chemical biology · 2020Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
6 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Spatiotemporal control of transgene expression is of paramount importance in gene therapy. Here, we demonstrate the use of magnetic resonance temperature imaging (MRI)-guided, high-intensity focused ultrasound (HIFU) in combination with a heat-inducible promoter [heat shock protein 70 (HSP70)] for the in vivo spatiotemporal control of transgene activation. Local gene activation induced by moderate hyperthermia in a transgenic mouse expressing luciferase under the control of the HSP70 promoter showed a high similarity between the local temperature distribution in vivo and the region emitting light. Modulation of gene expression is possible by changing temperature, duration, and location of regional heating. Mild heating protocols (2 min at 43 degrees C) causing no tissue damage were sufficient for significant gene activation. The HSP70 promoter was shown to be induced by the local temperature increase and not by the mechanical effects of ultrasound. Therefore, the combination of MRI-guided HIFU heating and transgenes under control of heat-inducible HSP promoter provides a direct, noninvasive, spatial control of gene expression via local hyperthermia.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.