ArticleProceedings of the National Academy of Sciences of the United States of America2009
Chloroplast protein targeting involves localized translation in Chlamydomonas.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 33 papers.
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33 citing papers in PubMed, 86 citations in OpenAlex.
- Ancient reticulation, incomplete lineage sorting and the evolution of the pyrenoid at the dawn of hornwort diversification.Annals of botany · 2025Article
- Chloroplast biogenesis involves spatial coordination of nuclear and organellar gene expression in Chlamydomonas.Plant physiology · 2024Article
- A prion-like domain is required for phase separation and chloroplast RNA processing during cold acclimation in Arabidopsis.The Plant cell · 2024Article
- Assembly and Repair of Photosystem II inPlants (Basel, Switzerland) · 2024Review
- Locations of membrane protein production in a cyanobacterium.Journal of bacteriology · 2023Article
- Increasing the Efficiency of the Accumulation of Recombinant Proteins in Plant Cells: The Role of Transport Signal Peptides.Plants (Basel, Switzerland) · 2022Review
- Fast and global reorganization of the chloroplast protein biogenesis network during heat acclimation.The Plant cell · 2022Article
- A recombineering pipeline to clone large and complex genes in Chlamydomonas.The Plant cell · 2021Article
- mRNA localization, reaction centre biogenesis and thylakoid membrane targeting in cyanobacteria.Nature plants · 2020Article
- Co-Translational Protein Folding and Sorting in Chloroplasts.Plants (Basel, Switzerland) · 2020Review
- Coupled Transcription-Translation in Prokaryotes: An Old Couple With New Surprises.Frontiers in microbiology · 2020Review
- Photosystem Biogenesis Is Localized to the Translation Zone in the Chloroplast of Chlamydomonas.The Plant cell · 2019Article
- Article
- Chloroplast nucleoids as a transformable network revealed by live imaging with a microfluidic device.Communications biology · 2018Article
- Structural and molecular comparison of bacterial and eukaryotic trigger factors.Scientific reports · 2017Article
- Stable Membrane-Association of mRNAs in Etiolated, Greening and Mature Plastids.International journal of molecular sciences · 2017Article
- Pyrenoid loss in Chlamydomonas reinhardtii causes limitations in CO2 supply, but not thylakoid operating efficiency.Journal of experimental botany · 2017Article
- Thylakoid Membrane Architecture inThe Plant cell · 2016Article
- Organization of chlorophyll biosynthesis and insertion of chlorophyll into the chlorophyll-binding proteins in chloroplasts.Photosynthesis research · 2015Review
- Genome-wide analysis of thylakoid-bound ribosomes in maize reveals principles of cotranslational targeting to the thylakoid membrane.Proceedings of the National Academy of Sciences of the United States of America · 2015Article
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The compartmentalization of eukaryotic cells requires that newly synthesized proteins be targeted to the compartments in which they function. In chloroplasts, a few thousand proteins function in photosynthesis, expression of the chloroplast genome, and other processes. Most chloroplast proteins are synthesized in the cytoplasm, imported, and then targeted to a specific chloroplast compartment. The remainder are encoded by the chloroplast genome, synthesized within the organelle, and targeted by mechanisms that are only beginning to be elucidated. We used fluorescence confocal microscopy to explore the targeting mechanisms used by several chloroplast proteins in the green alga Chlamydomonas. These include the small subunit of ribulose bisphosphate carboxylase (rubisco) and the light-harvesting complex II (LHCII) subunits, which are imported from the cytoplasm, and 2 proteins synthesized in the chloroplast: the D1 subunit of photosystem II and the rubisco large subunit. We determined whether the targeting of each protein involves localized translation of the mRNA that encodes it. When this was the case, we explored whether the targeting sequence was in the nascent polypeptide or in the mRNA, based on whether the localization was translation-dependent or -independent, respectively. The results reveal 2 novel examples of targeting by localized translation, in LHCII subunit import and the targeting of the rubisco large subunit to the pyrenoid. They also demonstrate examples of each of the three known mechanisms-posttranslational, cotranslational (signal recognition particle-mediated), and mRNA-based-in the targeting of specific chloroplast proteins. Our findings can help guide the exploration of these pathways at the biochemical level.
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