ArticleProceedings of the National Academy of Sciences of the United States of America2009
Electron microscopy of whole cells in liquid with nanometer resolution.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 98 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
98 citing papers in PubMed, 490 citations in OpenAlex.
- Live Imaging of Silver Nanostructures Electrochemically Dissolving at Open-Circuit Potential.Small (Weinheim an der Bergstrasse, Germany) · 2026Article
- Real-Time Visualization of Calcium Phosphate Formation on Titanium Dioxide Nanoparticles Using Liquid Transmission Electron Microscopy.Small (Weinheim an der Bergstrasse, Germany) · 2025Article
- Photoelectron spectroscopic operando studies of surface of nanoparticles in liquid or gas at bar pressure with an X-ray photoelectron spectrometer.Nature protocols · 2025Review
- Advancing Cryo-EM and Cryo-ET through Innovation in Sample Carriers: A Perspective.Analytical chemistry · 2025Review
- Imaging of Hydrated and Living Cells in Transmission Electron Microscope: Summary, Challenges, and Perspectives.ACS nano · 2025Review
- In Situ Transmission Electron Microscopy of Electrocatalyst Materials: Proposed Workflows, Technical Advances, Challenges, and Lessons Learned.Small methods · 2025Article
- Tutorial on In Situ andACS nano · 2024Review
- Advanced environmental scanning electron microscopy reveals natural surface nano-morphology of condensed mitotic chromosomes in their native state.Scientific reports · 2024Article
- Electron videography of a lipid-protein tango.Science advances · 2024Article
- Ultrathin silicon nitride microchip for in situ/operando microscopy with high spatial resolution and spectral visibility.Science advances · 2024Article
- Review
- Facile hermetic TEM grid preparation for molecular imaging of hydrated biological samples at room temperature.Nature communications · 2023Article
- Upper critical solution temperature polymer assemblies via variable temperature liquid phase transmission electron microscopy and liquid resonant soft X-ray scattering.Nature communications · 2023Article
- Dose-efficient scanning Compton X-ray microscopy.Light, science & applications · 2023Article
- Time-resolved transmission electron microscopy for nanoscale chemical dynamics.Nature reviews. Chemistry · 2023Review
- Facile hermetic TEM grid preparation for molecular imaging of hydrated biological samples at room temperature.Research square · 2023Article
- Article
- Differentiating Trypanosoma cruzi in a Host Mammalian Cell Imaged in Aqueous Liquid by Atmospheric Scanning Electron Microscopy.Microbiology spectrum · 2022Article
- Article
- Review
38 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors at 2 institutions in 1 country.
Funding
Abstract
Single gold-tagged epidermal growth factor (EGF) molecules bound to cellular EGF receptors of fixed fibroblast cells were imaged in liquid with a scanning transmission electron microscope (STEM). The cells were placed in buffer solution in a microfluidic device with electron transparent windows inside the vacuum of the electron microscope. A spatial resolution of 4 nm and a pixel dwell time of 20 micros were obtained. The liquid layer was sufficiently thick to contain the cells with a thickness of 7 +/- 1 microm. The experimental findings are consistent with a theoretical calculation. Liquid STEM is a unique approach for imaging single molecules in whole cells with significantly improved resolution and imaging speed over existing methods.
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What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.