ArticleMolecular plant pathology2009
Identification of virulence genes in Fusarium oxysporum f. sp. lycopersici by large-scale transposon tagging.
Article in Molecular plant pathology, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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Who cites it
22 citing papers in PubMed, 42 citations in OpenAlex.
- Advances in the detection technology of vegetable soil borne fungi and bacteria.Frontiers in microbiology · 2024Review
- Article
- Constitutive activation of TORC1 signalling attenuates virulence in the cross-kingdom fungal pathogen Fusarium oxysporum.Molecular plant pathology · 2023Article
- Unlocking the magic in mycelium: Using synthetic biology to optimize filamentous fungi for biomanufacturing and sustainability.Materials today. Bio · 2023Article
- Review
- Fusarium oxysporum f. sp. lycopersici CCurrent genetics · 2019Article
- Three Fusarium oxysporum mitogen-activated protein kinases (MAPKs) have distinct and complementary roles in stress adaptation and cross-kingdom pathogenicity.Molecular plant pathology · 2017Article
- Random mutagenesis analysis and identification of a novel CScientific reports · 2017Article
- A silver bullet in a golden age of functional genomics: the impact ofFungal biology and biotechnology · 2017Review
- The transmembrane protein Sho1 cooperates with the mucin Msb2 to regulate invasive growth and plant infection in Fusarium oxysporum.Molecular plant pathology · 2015Article
- Plant defense response against Fusarium oxysporum and strategies to develop tolerant genotypes in banana.Planta · 2014Review
- Identification and regulation of fusA, the polyketide synthase gene responsible for fusarin production in Fusarium fujikuroi.Applied and environmental microbiology · 2012Article
- The Top 10 fungal pathogens in molecular plant pathology.Molecular plant pathology · 2012Review
- The velvet gene, FgVe1, affects fungal development and positively regulates trichothecene biosynthesis and pathogenicity in Fusarium graminearum.Molecular plant pathology · 2012Article
- Fungal pathogenicity genes in the age of 'omics'.Molecular plant pathology · 2011Review
- The membrane mucin Msb2 regulates invasive growth and plant infection in Fusarium oxysporum.The Plant cell · 2011Article
- The bZIP transcription factor MeaB mediates nitrogen metabolite repression at specific loci.Eukaryotic cell · 2010Article
- A nitrogen response pathway regulates virulence functions in Fusarium oxysporum via the protein kinase TOR and the bZIP protein MeaB.The Plant cell · 2010Article
- The two-component histidine kinase Fhk1 controls stress adaptation and virulence of Fusarium oxysporum.Molecular plant pathology · 2010Article
- Proteomics of plant pathogenic fungi.Journal of biomedicine & biotechnology · 2010Review
Corrections and comments
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Authors and funding
8 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Forward genetic screens are efficient tools for the dissection of complex biological processes, such as fungal pathogenicity. A transposon tagging system was developed in the vascular wilt fungus Fusarium oxysporum f. sp. lycopersici by inserting the novel modified impala element imp160::gfp upstream of the Aspergillus nidulans niaD gene, followed by transactivation with a constitutively expressed transposase. A collection of 2072 Nia(+) revertants was obtained from reporter strain T12 and screened for alterations in virulence, using a rapid assay for invasive growth on apple slices. Seven strains exhibited reduced virulence on both apple slices and intact tomato plants. Five of these were true revertants showing the re-insertion of imp160::gfp within or upstream of predicted coding regions, whereas the other two showed either excision without re-insertion or no excision. Linkage between imp160::gfp insertion and virulence phenotype was determined in four transposon-tagged loci using targeted deletion in the wild-type strain. Knockout mutants in one of the genes, FOXG_00016, displayed significantly reduced virulence, and complementation of the original revertant with the wild-type FOXG_00016 allele fully restored virulence. FOXG_00016 has homology to the velvet gene family of A. nidulans. The high rate of untagged virulence mutations in the T12 reporter strain appears to be associated with increased genetic instability, possibly as a result of the transactivation of endogenous transposable elements by the constitutively expressed transposase.
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