ArticleFree radical biology & medicine2009
Immuno-spin trapping of protein and DNA radicals: "tagging" free radicals to locate and understand the redox process.
Article in Free radical biology & medicine, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 29 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
29 citing papers in PubMed, 62 citations in OpenAlex.
- Trapping DNA Radicals With DMPO Reduces Hypochlorous Acid-Induced 8-oxo-7,8-dihydro-2'-deoxyguanosine and Mutagenesis in Lung Epithelial Cells.Oxidative medicine and cellular longevity · 2025Article
- Nitroxyl Radical as a Theranostic Contrast Agent in Magnetic Resonance Redox Imaging.Antioxidants & redox signaling · 2022Article
- Free Radical-Mediated Protein Radical Formation in Differentiating Monocytes.International journal of molecular sciences · 2021Article
- Selective Chemical Functionalization at N6-Methyladenosine Residues in DNA Enabled by Visible-Light-Mediated Photoredox Catalysis.Journal of the American Chemical Society · 2020Article
- Article
- Co-stimulation of LPARStem cell research & therapy · 2018Article
- The nitrone spin trap 5,5-dimethyl-1-pyrroline N-oxide dampens lipopolysaccharide-induced transcriptomic changes in macrophages.Inflammation research : official journal of the European Histamine Research Society ... [et al.] · 2018Article
- Switch of Mitochondrial Superoxide Dismutase into a Prooxidant Peroxidase in Manganese-Deficient Cells and Mice.Cell chemical biology · 2018Article
- Assessing long-term neuroinflammatory responses to encephalopathy using MRI approaches in a rat endotoxemia model.GeroScience · 2018Article
- Immuno-Spin Trapping-Based Detection of Oxidative Modifications in Cardiomyocytes and Coronary Endothelium in the Progression of Heart Failure in Tgαq*44 Mice.Frontiers in immunology · 2018Article
- Use of a cocktail of spin traps for fingerprinting large range of free radicals in biological systems.PloS one · 2017Article
- Measurement of Reactive Oxygen Species, Reactive Nitrogen Species, and Redox-Dependent Signaling in the Cardiovascular System: A Scientific Statement From the American Heart Association.Circulation research · 2016Review
- Imaging free radicals in organelles, cells, tissue, and in vivo with immuno-spin trapping.Redox biology · 2016Review
- Reactive Oxygen Species: A Key Hallmark of Cardiovascular Disease.Advances in medicine · 2016Review
- Immuno-spin trapping detection of antioxidant/pro-oxidant properties of zinc or selenium on DNA and protein radical formation via hydrogen peroxide.Molecular and cellular biochemistry · 2015Article
- Iron incorporation into MnSOD A (bacterial Mn-dependent superoxide dismutase) leads to the formation of a peroxidase/catalase implicated in oxidative damage to bacteria.Biochimica et biophysica acta · 2015Article
- Radicalization of Glyceraldehyde-3-Phosphate Dehydrogenase by HOCl in Living Cells.Enzyme engineering (Los Angeles, Calif.) · 2015Article
- Absence of an effect of vitamin E on protein and lipid radical formation during lipoperoxidation of LDL by lipoxygenase.Free radical biology & medicine · 2014Article
- Immuno-spin trapping from biochemistry to medicine: advances, challenges, and pitfalls. Focus on protein-centered radicals.Biochimica et biophysica acta · 2014Review
- Methods for detection of mitochondrial and cellular reactive oxygen species.Antioxidants & redox signaling · 2014Review
Corrections and comments
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Authors and funding
10 authors at 3 institutions in 1 country.
Funding
Abstract
Biomolecule-centered radicals are intermediate species produced during both reversible (redox modulation) and irreversible (oxidative stress) oxidative modification of biomolecules. These oxidative processes must be studied in situ and in real time to understand the molecular mechanism of cell adaptation or death in response to changes in the extracellular environment. In this regard, we have developed and validated immuno-spin trapping to tag the redox process, tracing the oxidatively generated modification of biomolecules, in situ and in real time, by detecting protein- and DNA-centered radicals. The purpose of this methods article is to introduce and update the basic methods and applications of immuno-spin trapping for the study of redox biochemistry in oxidative stress and redox regulation. We describe in detail the production, detection, and location of protein and DNA radicals in biochemical systems, cells, and tissues, and in the whole animal as well, by using immuno-spin trapping with the nitrone spin trap 5,5-dimethyl-1-pyrroline N-oxide.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.