ArticleDevelopmental biology2009
Identification of an ancient Bmp4 mesoderm enhancer located 46 kb from the promoter.
Article in Developmental biology, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
23 citing papers in PubMed.
- HOXA5-driven transcriptional program in lung development.Developmental biology · 2025Article
- The case against simplistic genetic explanations of evolution.Development (Cambridge, England) · 2024Review
- Next-generation plasmids for transgenesis in zebrafish and beyond.Development (Cambridge, England) · 2023Article
- Joint disease-specificity at the regulatory base-pair level.Nature communications · 2021Article
- Bidirectional Wnt signaling between endoderm and mesoderm confers tracheal identity in mouse and human cells.Nature communications · 2020Article
- A conserved regulatory program initiates lateral plate mesoderm emergence across chordates.Nature communications · 2019Article
- Lsh/HELLS regulates self-renewal/proliferation of neural stem/progenitor cells.Scientific reports · 2017Article
- Review
- Dynamics and cellular localization of Bmp2, Bmp4, and Noggin transcription in the postnatal mouse skeleton.Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research · 2015Article
- A nonsynonymous mutation in the transcriptional regulator lbh is associated with cichlid craniofacial adaptation and neural crest cell development.Molecular biology and evolution · 2014Article
- Identification of the control region of pancreatic expression of Bmp4 in vitro and in vivo.PloS one · 2013Article
- UG4 enhancer-driven GATA-2 and bone morphogenetic protein 4 complementation remedies the CAKUT phenotype in Gata2 hypomorphic mutant mice.Molecular and cellular biology · 2012Article
- Diverse functions of distal regulatory elements at the IFNG locus.Journal of immunology (Baltimore, Md. : 1950) · 2012Article
- An evolutionarily conserved enhancer regulates Bmp4 expression in developing incisor and limb bud.PloS one · 2012Article
- Mutation of Rubie, a novel long non-coding RNA located upstream of Bmp4, causes vestibular malformation in mice.PloS one · 2012Article
- Msx1 mutations: how do they cause tooth agenesis?Journal of dental research · 2011Article
- Establishment and characterization of a transgenic mouse model for in vivo imaging of Bmp4 expression in the pancreas.PloS one · 2011Article
- Regulation of bmp4 expression in odontogenic mesenchyme: from simple to complex.Cells, tissues, organs · 2011Article
- BMPs and FGFs target Notch signalling via jagged 2 to regulate tooth morphogenesis and cytodifferentiation.Development (Cambridge, England) · 2010Article
- Article
Corrections and comments
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Authors and funding
3 authors.
Funding
Abstract
Bone morphogenetic protein 4 (Bmp4) is a multi-functional, developmentally regulated gene that is essential for mouse development, as most Bmp4-null mouse embryos die at the onset of gastrulation and fail to develop mesoderm. Little is known about the transcriptional regulation of Bmp4. To identify potential long-range cis-regulatory elements that direct its complex spatiotemporal expression patterns, we surveyed the mouse Bmp4 locus using two overlapping bacterial artificial chromosome (BAC) reporter transgenes. Our findings indicate that tissue-specific cis-regulatory elements reside greater than 28 kb 5' or 3' to the mouse Bmp4 transcription unit. In addition, comparative analyses identified three noncoding evolutionarily conserved regions (ECRs), spaced around the gene and conserved from mammals to fish, that are maintained in a syntenic group across vertebrates. Deletion of one of these conserved sequences (ECR2) from a BAC transgene revealed a tissue-specific requirement for ECR2 in driving Bmp4 expression in extraembryonic and embryonic mesoderm. Furthermore, a 467 bp mouse sequence containing ECR2 reproducibly directed lacZ minigene expression in mesoderm. Taken together, this shows that an ancient, mesoderm-specific cis-regulatory element resides nearly 50 kb 5' to mouse Bmp4.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.