ArticleMolecular vision2009
Apoptosis induction by oxidized glycated LDL in human retinal capillary pericytes is independent of activation of MAPK signaling pathways.
Article in Molecular vision, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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Who cites it
23 citing papers in PubMed, 30 citations in OpenAlex.
- Pericyte loss: a key factor inducing brain Aβ40 accumulation and neuronal degeneration in cerebral amyloid angiopathy.Experimental brain research · 2025Article
- Circulating Sphingolipids in Insulin Resistance, Diabetes and Associated Complications.International journal of molecular sciences · 2023Review
- Potential impact of epicardial fat thickness, pentraxin-3, and high-sensitive C-reactive protein on the risk of non-proliferative diabetic retinopathy.Journal of diabetes and metabolic disorders · 2023Article
- The Effect of Hyperlipidemia on the Course of Diabetic Retinopathy-Literature Review.Journal of clinical medicine · 2022Review
- Emerging Roles of Dyslipidemia and Hyperglycemia in Diabetic Retinopathy: Molecular Mechanisms and Clinical Perspectives.Frontiers in endocrinology · 2021Review
- Effects of modified lipoproteins on human trophoblast cells: a role in pre-eclampsia in pregnancies complicated by diabetes.BMJ open diabetes research & care · 2021Article
- Salidroside Reduces High-Glucose-Induced Podocyte Apoptosis and Oxidative Stress via Upregulating Heme Oxygenase-1 (HO-1) Expression.Medical science monitor : international medical journal of experimental and clinical research · 2017Article
- Survival or death: a dual role for autophagy in stress-induced pericyte loss in diabetic retinopathy.Diabetologia · 2016Article
- Extravascular modified lipoproteins: a role in the propagation of diabetic retinopathy in a mouse model of type 1 diabetes.Diabetologia · 2016Article
- Cultured cells of the blood-brain barrier from apolipoprotein B-100 transgenic mice: effects of oxidized low-density lipoprotein treatment.Fluids and barriers of the CNS · 2015Article
- Immune complex formation in human diabetic retina enhances toxicity of oxidized LDL towards retinal capillary pericytes.Journal of lipid research · 2014Article
- Oxidative stress: implications for the development of diabetic retinopathy and antioxidant therapeutic perspectives.Oxidative medicine and cellular longevity · 2014Review
- Modified Lipoproteins in Diabetic Retinopathy: A Local Action in the Retina.Journal of clinical & experimental ophthalmology · 2013Article
- Effects of modified LDL and HDL on retinal pigment epithelial cells: a role in diabetic retinopathy?Diabetologia · 2013Article
- Surfactin-induced apoptosis through ROS-ERS-Ca2+-ERK pathways in HepG2 cells.Cell biochemistry and biophysics · 2013Article
- Mechanisms of modified LDL-induced pericyte loss and retinal injury in diabetic retinopathy.Diabetologia · 2012Article
- Globular adiponectin protects H9c2 cells from palmitate-induced apoptosis via Akt and ERK1/2 signaling pathways.Lipids in health and disease · 2012Article
- Oxidative and endoplasmic reticulum stresses mediate apoptosis induced by modified LDL in human retinal Müller cells.Investigative ophthalmology & visual science · 2012Article
- Tyrosine nitration of prostacyclin synthase is associated with enhanced retinal cell apoptosis in diabetes.The American journal of pathology · 2011Article
- Pericytes control key neurovascular functions and neuronal phenotype in the adult brain and during brain aging.Neuron · 2010Article
Corrections and comments
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
Abstract
backgroundPericyte loss is a cardinal feature of early diabetic retinopathy. We previously reported that highly oxidized-glycated low density lipoprotein (HOG-LDL) induces pericyte apoptosis in vitro. In this study, we investigated the role of the mitogen-activated protein kinase (MAPK) signaling pathways in HOG-LDL-induced apoptosis in human pericytes.
methodsHuman retinal capillary pericytes (HRCP) were exposed to native LDL (N-LDL) and HOG-LDL, and apoptosis was measured using flow cytometry. Time- and dose-dependent responses of extracellular signal-regulated kinase (ERK), p38, and Jun N-terminal kinase (JNK) following exposure to N-LDL or HOG-LDL were determined using western blotting. U0126 (ERK inhibitor), SB203580 (p38 inhibitor), and SP600125 (JNK inhibitor) were used to determine the role of MAPK signaling in HOG-LDL-induced apoptosis.
resultsHOG-LDL induced apoptosis in HRCP in a dose-dependent manner at concentrations from 5 to 50 mg/l, with a constant effect from 50 to 200 mg/l. When compared to serum-free medium (SFM), this effect of HOG-LDL was found to be significant at all doses above 10 mg/l. In contrast, N-LDL at 200 mg/l did not induce apoptosis compared with SFM. Exposure to N-LDL versus HOG-LDL induced similar phosphorylation of ERK, p38, and JNK, peaking at 5 min, with similar dose-dependent responses up to 25 mg/l that were constant from 25 to 100 mg/l. Blocking of the ERK, p38, and JNK pathways did not inhibit pericyte apoptosis induced by HOG-LDL.
conclusionsOur data suggest that apoptosis induced by HOG-LDL in HRCP is independent of the activation of MAPK signaling pathways.
Indexed as
Identifiers
19158958PMC2628314W2111489239What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.