ArticleMolecular biology of the cell2009
Myosin 2 maintains an open exocytic fusion pore in secretory epithelial cells.
Article in Molecular biology of the cell, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 34 papers.
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Who cites it
34 citing papers in PubMed, 50 citations in OpenAlex.
- PI(4,5)PBiochimica et biophysica acta. Molecular and cell biology of lipids · 2025Review
- Fusion pore dynamics of large secretory vesicles define a distinct mechanism of exocytosis.The Journal of cell biology · 2023Article
- Sequential compound fusion and kiss-and-run mediate exo- and endocytosis in excitable cells.Science advances · 2022Article
- Real-time visualization of exo- and endocytosis membrane dynamics with confocal and super-resolution microscopy.STAR protocols · 2022Article
- Brain-derived neurotrophic factor regulates LYN kinase-mediated myosin light chain kinase activation to modulate nonmuscle myosin II activity in hippocampal neurons.The Journal of biological chemistry · 2022Article
- Oncogenic Kras-Mediated Cytokine CCL15 Regulates Pancreatic Cancer Cell Migration and Invasion through ROS.Cancers · 2022Article
- Actin and Myosin in Non-Neuronal Exocytosis.Cells · 2020Review
- Toward a unified picture of the exocytotic fusion pore.FEBS letters · 2018Review
- The Actomyosin Cytoskeleton Drives Micron-Scale Membrane Remodeling In Vivo Via the Generation of Mechanical Forces to Balance Membrane Tension Gradients.BioEssays : news and reviews in molecular, cellular and developmental biology · 2018Review
- Concerted actions of distinct nonmuscle myosin II isoforms drive intracellular membrane remodeling in live animals.The Journal of cell biology · 2017Article
- ALPK1 phosphorylates myosin IIA modulating TNF-α trafficking in gout flares.Scientific reports · 2016Article
- Orchestrated content release from Drosophila glue-protein vesicles by a contractile actomyosin network.Nature cell biology · 2016Article
- Actin depolymerisation and crosslinking join forces with myosin II to contract actin coats on fused secretory vesicles.Journal of cell science · 2015Article
- Synaptotagmin-7 links fusion-activated Ca²⁺ entry and fusion pore dilation.Journal of cell science · 2014Article
- A new role for myosin II in vesicle fission.PloS one · 2014Article
- The cortical acto-Myosin network: from diffusion barrier to functional gateway in the transport of neurosecretory vesicles to the plasma membrane.Frontiers in endocrinology · 2013Review
- Multiple roles for the actin cytoskeleton during regulated exocytosis.Cellular and molecular life sciences : CMLS · 2013Review
- Non-muscle myosin IIA is involved in focal adhesion and actin remodelling controlling glucose-stimulated insulin secretion.Diabetologia · 2013Article
- Nonmuscle myosin-2: mix and match.Cellular and molecular life sciences : CMLS · 2013Review
- α-catenin and IQGAP regulate myosin localization to control epithelial tube morphogenesis in Dictyostelium.Developmental cell · 2012Article
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Many studies have implicated F-actin and myosin 2 in the control of regulated secretion. Most recently, evidence suggests a role for the microfilament network in regulating the postfusion events of vesicle dynamics. This is of potential importance as postfusion behavior can influence the loss of vesicle content and may provide a new target for drug therapy. We have investigated the role of myosin 2 in regulating exocytosis in secretory epithelial cells by using novel assays to determine the behavior of the fusion pore in individual granules. We immunolocalize myosin 2A to the apical region of pancreatic acinar cells, suggesting it is this isoform that plays a role in granule exocytosis. We further show myosin 2 phosphorylation increased on cell stimulation, consistent with a regulatory role in secretion. Importantly, in a single-cell, single-granule secretion assay, neither the myosin 2 inhibitor (-)-blebbistatin nor the myosin light chain kinase inhibitor ML-9 had any effect on the numbers of granules stimulated to fuse after cell stimulation. These data indicate that myosin 2, if it has any action on secretion, must be targeting postfusion granule behavior. This interpretation is supported by direct study of fusion pore opening in which we show that (-)-blebbistatin and ML-9 promote fusion pore closure and decrease fusion pore lifetimes. Our work now adds to a growing body of evidence showing that myosin 2 is an essential regulator of postfusion granule behavior. In particular, in the case of the secretory epithelial cells, myosin 2 activity is necessary to maintain fusion pore opening.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.