ArticleThe Journal of neuroscience : the official journal of the Society for Neuroscience2009
Protein synthesis in distal axons is not required for growth cone responses to guidance cues.
Article in The Journal of neuroscience : the official journal of the Society for Neuroscience, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 55 papers.
What it found
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Who cites it
55 citing papers in PubMed, 79 citations in OpenAlex.
- The Axonal Actin Filament Cytoskeleton: Structure, Function, and Relevance to Injury and Degeneration.Molecular neurobiology · 2024Review
- Actin cytoskeletal dynamics do not impose an energy drain on growth cone bioenergetics.Journal of cell science · 2023Article
- Mito-FUNCAT-FACS reveals cellular heterogeneity in mitochondrial translation.RNA (New York, N.Y.) · 2022Article
- SARM1 Suppresses Axon Branching Through Attenuation of Axonal Cytoskeletal Dynamics.Frontiers in molecular neuroscience · 2022Article
- SPAAC Pulse-Chase: A Novel Click Chemistry-Based Method to Determine the Half-Life of Cellular Proteins.Frontiers in cell and developmental biology · 2021Article
- Mid-infrared metabolic imaging with vibrational probes.Nature methods · 2020Article
- Local Translation in Growth Cones and Presynapses, Two Axonal Compartments for Local Neuronal Functions.Biomolecules · 2020Review
- Article
- Axon guidance pathways and the control of gene expression.Developmental dynamics : an official publication of the American Association of Anatomists · 2018Review
- CSPGs inhibit axon branching by impairing mitochondria-dependent regulation of actin dynamics and axonal translation.Developmental neurobiology · 2017Article
- Sonic Hedgehog Guides Axons via Zipcode Binding Protein 1-Mediated Local Translation.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2017Article
- Article
- Intra-axonal protein synthesis in development and beyond.International journal of developmental neuroscience : the official journal of the International Society for Developmental Neuroscience · 2016Review
- Glutamate Stimulates Local Protein Synthesis in the Axons of Rat Cortical Neurons by Activating α-Amino-3-hydroxy-5-methyl-4-isoxazolepropionic Acid (AMPA) Receptors and Metabotropic Glutamate Receptors.The Journal of biological chemistry · 2015Article
- Isolation and culture of dissociated sensory neurons from chick embryos.Journal of visualized experiments : JoVE · 2014Article
- Zebrafish embryonic neurons transport messenger RNA to axons and growth cones in vivo.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2013Article
- Teaching old NCATs new tricks: using non-canonical amino acid tagging to study neuronal plasticity.Current opinion in chemical biology · 2013Review
- Cell biology in neuroscience: RNA-based mechanisms underlying axon guidance.The Journal of cell biology · 2013Review
- Vibrational imaging of newly synthesized proteins in live cells by stimulated Raman scattering microscopy.Proceedings of the National Academy of Sciences of the United States of America · 2013Article
- Intra-axonal synthesis of eukaryotic translation initiation factors regulates local protein synthesis and axon growth in rat sympathetic neurons.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2013Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
Recent evidence suggests that growth cone responses to guidance cues require local protein synthesis. Using chick neurons, we investigated whether protein synthesis is required for growth cones of several types to respond to guidance cues. First, we found that global inhibition of protein synthesis stops axonal elongation after 2 h. When protein synthesis inhibitors were added 15 min before adding guidance cues, we found no changes in the typical responses of retinal, sensory, and sympathetic growth cones. In the presence of cycloheximide or anisomycin, ephrin-A2, slit-3, and semaphorin3A still induced growth cone collapse and loss of actin filaments, nerve growth factor (NGF) and neurotrophin-3 still induced growth cone protrusion and increased filamentous actin, and sensory growth cones turned toward an NGF source. In compartmented chambers that separated perikarya from axons, axons grew for 24-48 h in the presence of cycloheximide and responded to negative and positive cues. Our results indicate that protein synthesis is not strictly required in the mechanisms for growth cone responses to many guidance cues. Differences between our results and other studies may exist because of different cellular metabolic levels in in vitro conditions and a difference in when axonal functions become dependent on local protein synthesis.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.