ArticleOsteoarthritis and cartilage2009
Central and peripheral region tibial plateau chondrocytes respond differently to in vitro dynamic compression.
Article in Osteoarthritis and cartilage, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
21 citing papers in PubMed, 49 citations in OpenAlex.
- Compressive stress-driven mechanosignaling in chondrocytes: From molecular mechanism to scaffold engineering and translational opportunities.Materials today. Bio · 2026Review
- Influence of the Mechanical Environment on the Regeneration of Osteochondral Defects.Frontiers in bioengineering and biotechnology · 2021Review
- Article
- Mechanically stimulated biomarkers signal cartilage changes over 5 years consistent with disease progression in medial knee osteoarthritis patients.Journal of orthopaedic research : official publication of the Orthopaedic Research Society · 2018Article
- Men and Women Differ in the Biochemical Composition of Platelet-Rich Plasma.The American journal of sports medicine · 2018Article
- Biochemical markers of cartilage metabolism are associated with walking biomechanics 6-months following anterior cruciate ligament reconstruction.Journal of orthopaedic research : official publication of the Orthopaedic Research Society · 2017Article
- Early Changes in Knee Center of Rotation During Walking After Anterior Cruciate Ligament Reconstruction Correlate With Later Changes in Patient-Reported Outcomes.The American journal of sports medicine · 2017Article
- Design and Validation of Equiaxial Mechanical Strain Platform, EQUicycler, for 3D Tissue Engineered Constructs.BioMed research international · 2017Article
- Mechanoresponsive musculoskeletal tissue differentiation of adipose-derived stem cells.Biomedical engineering online · 2016Review
- Cartilage Strain Distributions Are Different Under the Same Load in the Central and Peripheral Tibial Plateau Regions.Journal of biomechanical engineering · 2015Article
- A systems view of risk factors for knee osteoarthritis reveals insights into the pathogenesis of the disease.Annals of biomedical engineering · 2015Review
- The nature of in vivo mechanical signals that influence cartilage health and progression to knee osteoarthritis.Current rheumatology reports · 2014Review
- The regional sensitivity of chondrocyte gene expression to coactive mechanical load and exogenous TNF-α stimuli.Journal of biomechanical engineering · 2014Article
- Immature articular cartilage and subchondral bone covered by menisci are potentially susceptive to mechanical load.BMC musculoskeletal disorders · 2014Article
- Patterns of femoral cartilage thickness are different in asymptomatic and osteoarthritic knees and can be used to detect disease-related differences between samples.Journal of biomechanical engineering · 2013Article
- Changes in dynamic medial tibiofemoral contact mechanics and kinematics after injury of the anterior cruciate ligament: a cadaveric model.Proceedings of the Institution of Mechanical Engineers. Part H, Journal of engineering in medicine · 2013Article
- The pain-relieving qualities of exercise in knee osteoarthritis.Open access rheumatology : research and reviews · 2013Review
- Differential limb loading in miniature pigs (Sus scrofa domesticus): a test of chondral modeling theory.The Journal of experimental biology · 2012Article
- Dynamic loading enhances integrative meniscal repair in the presence of interleukin-1.Osteoarthritis and cartilage · 2010Article
- Increased tibiofemoral cartilage contact deformation in patients with anterior cruciate ligament deficiency.Arthritis and rheumatism · 2009Article
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Authors and funding
4 authors at 2 institutions in 1 country.
Funding
Abstract
objectiveThe objective of this study was to test the hypotheses that chondrocytes from distinct regions of the porcine tibial plateau: (1) display region-specific baseline gene expression, and (2) respond differently to in vitro mechanical loading.
methodsArticular cartilage explants were obtained from central (not covered by meniscus) and peripheral (covered by meniscus) regions of porcine tibial plateaus. For baseline gene expression analysis, samples were snap frozen. To determine the effect of mechanical loading, central and peripheral region explants were exposed to equivalent dynamic compression (0-100 kPa) and compared to site-matched free-swelling controls (FSCs). mRNA levels for type II collagen (CII), aggrecan (AGGR), matrix metalloproteinase 1 (MMP-1), MMP-3, MMP-13, A disintegrin and metalloproteinase with thrombospondin motifs 4 (ADAM-TS4), ADAM-TS5, tissue inhibitor of metalloproteinases 1 (TIMP-1), TIMP-2, and tumor necrosis factor alpha (TNFalpha) were quantified using real time polymerase chain reaction (RT-PCR).
resultsAt baseline, mRNA levels for the structural proteins CII and AGGR were approximately twofold greater in the central region compared with peripheral region explants. In vitro dynamic compression strongly affected expression levels for CII, AGGR, MMP-3, and TIMP-2 relative to FSCs. Response differed significantly by region, with greater upregulation of CII, AGGR, and MMP-3 in central region explants.
conclusionsChondrocytes from different regions of the porcine tibial plateau express mRNA for structural proteins at different levels and respond to equivalent in vitro mechanical loading with distinctive changes in gene expression. These regional biological variations appear to be related to the local mechanical environment in the normal joint, and thus may indicate a sensitivity of the joint to conditions that alter joint loading such as anterior cruciate ligament (ACL) injury, meniscectomy, or joint instability.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.