ArticleFunctional & integrative genomics2009
Matching the proteome to the genome: the microbody of penicillin-producing Penicillium chrysogenum cells.
Article in Functional & integrative genomics, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 42 papers.
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Who cites it
42 citing papers in PubMed, 90 citations in OpenAlex.
- Virulensome: a transient organelle mediating fungal virulence.Science China. Life sciences · 2026Article
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- Synthetic Biology in Natural Product Biosynthesis.Chemical reviews · 2025Review
- Targeting of Specialized Metabolites Biosynthetic Enzymes to Membranes and Vesicles by Posttranslational Palmitoylation: A Mechanism of Non-Conventional Traffic and Secretion of Fungal Metabolites.International journal of molecular sciences · 2024Review
- Review
- Subcellular localization of fungal specialized metabolites.Fungal biology and biotechnology · 2022Review
- Review
- Translating theFrontiers in cell and developmental biology · 2022Review
- Nonribosomal peptide synthetases and their biotechnological potential in Penicillium rubens.Journal of industrial microbiology & biotechnology · 2021Review
- Comparative genomics of Alexander Fleming's original Penicillium isolate (IMI 15378) reveals sequence divergence of penicillin synthesis genes.Scientific reports · 2020Article
- Omics Approaches Applied toGenes · 2020Review
- Transport systems, intracellular traffic of intermediates and secretion of β-lactam antibiotics in fungi.Fungal biology and biotechnology · 2020Review
- Synthetic control devices for gene regulation in Penicillium chrysogenum.Microbial cell factories · 2019Article
- Hansenula polymorpha Aat2p is targeted to peroxisomes via a novel Pex20p-dependent pathway.FEBS letters · 2018Article
- Genomic mutational analysis of the impact of the classical strain improvement program on β-lactam producing Penicillium chrysogenum.BMC genomics · 2015Article
- Fungal siderophore metabolism with a focus on Aspergillus fumigatus.Natural product reports · 2014Review
- Article
- Dual targeting of peroxisomal proteins.Frontiers in physiology · 2013Review
- Fungal siderophore biosynthesis is partially localized in peroxisomes.Molecular microbiology · 2013Article
- Intracellular targeting of ascomycetous catalase-peroxidases (KatG1s).Archives of microbiology · 2013Article
Corrections and comments
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Authors and funding
7 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
In the filamentous fungus Penicillium chrysogenum, microbodies are essential for penicillin biosynthesis. To better understand the role of these organelles in antibiotics production, we determined the matrix enzyme contents of P. chrysogenum microbodies. Using a novel in silico approach, we first obtained a catalogue of 200 P. chrysogenum proteins with putative microbody targeting signals (PTSs). This included two orthologs of proteins involved in cephalosporin biosynthesis, which we demonstrate to be bona fide microbody matrix constituents. Subsequently, we performed a proteomics based inventory of P. chrysogenum microbody matrix proteins using nano-LC-MS/MS analysis. We identified 89 microbody proteins, 79 with a PTS, including the two known microbody-borne penicillin biosynthesis enzymes, isopenicillin N:acyl CoA acyltransferase and phenylacetyl-CoA ligase. Comparative analysis revealed that 69 out of 79 PTS proteins identified experimentally were in the reference list. A prominent microbody protein was identified as a novel fumarate reductase-cytochrome b5 fusion protein, which contains an internal PTS2 between the two functional domains. We show that this protein indeed localizes to P. chrysogenum microbodies.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.