ArticleMolecular systems biology2009
Dynamic map of protein interactions in the Escherichia coli chemotaxis pathway.
Article in Molecular systems biology, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 53 papers.
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Who cites it
53 citing papers in PubMed, 91 citations in OpenAlex.
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- Multiple ParA/MinD ATPases coordinate the positioning of disparate cargos in a bacterial cell.Nature communications · 2023Article
- Two antagonistic response regulators control Pseudomonas aeruginosa polarization during mechanotaxis.The EMBO journal · 2023Article
- FliL Differentially Interacts with Two Stator Systems To Regulate Flagellar Motor Output in Pseudomonas aeruginosa.Applied and environmental microbiology · 2022Article
- Real-time detection of response regulator phosphorylation dynamics in live bacteria.Proceedings of the National Academy of Sciences of the United States of America · 2022Article
- A Selective Tether Recruits Activated Response Regulator CheB to Its Chemoreceptor Substrate.mBio · 2021Article
- Functional determinants of a small protein controlling a broadly conserved bacterial sensor kinase.Journal of bacteriology · 2020Article
- C-terminal eYFP fusion impairsOpen biology · 2020Article
- The protein architecture of the endocytic coat analyzed by FRET microscopy.Molecular systems biology · 2020Article
- Ratiometric population sensing by a pump-probe signaling system in Bacillus subtilis.Nature communications · 2020Article
- A computational model for how cells choose temporal or spatial sensing during chemotaxis.PLoS computational biology · 2018Article
- Phenotypic diversity and temporal variability in a bacterial signaling network revealed by single-cell FRET.eLife · 2017Article
- Article
- Simultaneously measuring multiple protein interactions and their correlations in a cell by Protein-interactome Footprinting.Scientific reports · 2017Article
- Direct Correlation between Motile Behavior and Protein Abundance in Single Cells.PLoS computational biology · 2016Article
- Sugar Influx Sensing by the Phosphotransferase System of Escherichia coli.PLoS biology · 2016Article
- Article
- Bacterial protein networks: properties and functions.Nature reviews. Microbiology · 2015Review
Corrections and comments
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Authors and funding
2 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Protein-protein interactions play key roles in virtually all cellular processes, often forming complex regulatory networks. A powerful tool to study interactions in vivo is fluorescence resonance energy transfer (FRET), which is based on the distance-dependent energy transfer from an excited donor to an acceptor fluorophore. Here, we used FRET to systematically map all protein interactions in the chemotaxis signaling pathway in Escherichia coli, one of the most studied models of signal transduction, and to determine stimulation-induced changes in the pathway. Our FRET analysis identified 19 positive FRET pairs out of the 28 possible protein combinations, with 9 pairs being responsive to chemotactic stimulation. Six stimulation-dependent and five stimulation-independent interactions were direct, whereas other interactions were apparently mediated by scaffolding proteins. Characterization of stimulation-induced responses revealed an additional regulation through activity dependence of interactions involving the adaptation enzyme CheB, and showed complex rearrangement of chemosensory receptors. Our study illustrates how FRET can be efficiently employed to study dynamic protein networks in vivo.
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