ArticleRNA (New York, N.Y.)2009
Uncoupling of lin-14 mRNA and protein repression by nutrient deprivation in Caenorhabditis elegans.
Article in RNA (New York, N.Y.), 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
13 citing papers in PubMed, 22 citations in OpenAlex.
- Recent Molecular Genetic Explorations ofGenetics · 2018Article
- MicroRNA-Directed Cancer Therapies: Implications in Melanoma Intervention.The Journal of pharmacology and experimental therapeutics · 2018Review
- Neuronal function of the mRNA decapping complex determines survival ofOpen biology · 2017Article
- mRNA destabilization is the dominant effect of mammalian microRNAs by the time substantial repression ensues.Molecular cell · 2014Article
- Physiological stressors and invasive plant infections alter the small RNA transcriptome of the rice blast fungus, Magnaporthe oryzae.BMC genomics · 2013Article
- Article
- Conserved miRNAs are candidate post-transcriptional regulators of developmental arrest in free-living and parasitic nematodes.Genome biology and evolution · 2013Article
- Contributions of mRNA abundance, ribosome loading, and post- or peri-translational effects to temporal repression of C. elegans heterochronic miRNA targets.Genome research · 2012Article
- Effect of life history on microRNA expression during C. elegans development.RNA (New York, N.Y.) · 2011Article
- Regulation of lin-4 miRNA expression, organismal growth and development by a conserved RNA binding protein in C. elegans.Developmental biology · 2010Article
- MicroRNA functions in stress responses.Molecular cell · 2010Review
- miRNAs give worms the time of their lives: small RNAs and temporal control in Caenorhabditis elegans.Developmental dynamics : an official publication of the American Association of Anatomists · 2010Review
- Concordant regulation of translation and mRNA abundance for hundreds of targets of a human microRNA.PLoS biology · 2009Article
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
In animals, microRNAs (miRNAs), typically, pair to sites of partial complementarity in the 3'-untranslated regions (3'UTRs) of target genes. Regulation by miRNAs often results in down-regulation of target mRNA and protein expression by mechanisms that are yet to be fully elucidated. Additionally, changes in environmental conditions have been shown to influence miRNA function in some cell culture systems. Here, we report the effect of nutrient deprivation on regulation of an endogenous miRNA target in developing worms. In Caenorhabditis elegans, the lin-4 miRNA recognizes multiple sites in the lin-14 3'UTR and directs mRNA degradation and translational repression, but it is unclear how these processes are coupled. In this study, we demonstrate that nutrient deprivation results in loss of lin-14 mRNA, but not protein, repression. In worms removed from feeding conditions, lin-14 mRNA reaccumulates despite the continued expression of lin-4 miRNA. The relative increase in lin-14 mRNA levels during nutrient deprivation is less pronounced in genetic mutants lacking lin-4 miRNA or the lin-14 3'UTR target sites. In conclusion, regulation of lin-14 at the mRNA and protein levels can be uncoupled by changes in culture conditions, indicating that miRNA function can be modulated by environment in multicellular organisms. The awareness that endogenous miRNA pathways can be sensitive to environment is an important consideration for elucidating the mechanism used by miRNAs to regulate target mRNA and protein expression.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.