ArticleParticle and fibre toxicology2009
Genotoxic responses to titanium dioxide nanoparticles and fullerene in gpt delta transgenic MEF cells.
Article in Particle and fibre toxicology, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
17 citing papers in PubMed, 115 citations in OpenAlex.
- A Regulatory-Compliant Genotoxicity Study of a Mixture of C60 and C70 Fullerenes Dissolved in Olive Oil Using the Mammalian Micronucleus Test.Nanomaterials (Basel, Switzerland) · 2025Article
- Genotoxicity Evaluation of Titanium Dioxide Nanoparticles In Vivo and In Vitro: A Meta-Analysis.Toxics · 2023Review
- Nanoparticles for Topical Application in the Treatment of Skin Dysfunctions-An Overview of Dermo-Cosmetic and Dermatological Products.International journal of molecular sciences · 2022Review
- In vivo genotoxicity assessment of a multiwalled carbon nanotube in a mouse ex vivo culture.Genes and environment : the official journal of the Japanese Environmental Mutagen Society · 2022Article
- Safety assessment of titanium dioxide (E171) as a food additive.EFSA journal. European Food Safety Authority · 2021Article
- Modulation ofChemical research in toxicology · 2020Article
- Nanotechnologies in Food Science: Applications, Recent Trends, and Future Perspectives.Nano-micro letters · 2020Review
- Absence ofGenes and environment : the official journal of the Japanese Environmental Mutagen Society · 2017Article
- Health risk assessments of lithium titanate nanoparticles in rat liver cell model for its safe applications in nanopharmacology and nanomedicine.Cytotechnology · 2016Article
- Proteomic analysis of protein carbonylation: a useful tool to unravel nanoparticle toxicity mechanisms.Particle and fibre toxicology · 2015Article
- The potential for chemical mixtures from the environment to enable the cancer hallmark of sustained proliferative signalling.Carcinogenesis · 2015Review
- The importance of a validated standard methodology to define in vitro toxicity of nano-TiO2.Protoplasma · 2012Review
- Applications of nanotechnology in dermatology.The Journal of investigative dermatology · 2012Review
- Titanium dioxide in our everyday life; is it safe?Radiology and oncology · 2011Article
- Superparamagnetic iron oxide nanoparticles: promises for diagnosis and treatment of multiple sclerosis.ACS chemical neuroscience · 2011Review
- Current studies into the genotoxic effects of nanomaterials.Journal of nucleic acids · 2010Article
- Genotoxicity of nano/microparticles in in vitro micronuclei, in vivo comet and mutation assay systems.Particle and fibre toxicology · 2009Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors at 2 institutions in 2 countries.
Funding
Abstract
backgroundTitanium dioxide (TiO2) nanoparticles and fullerene (C60) are two attractive manufactured nanoparticles with great promise in industrial and medical applications. However, little is known about the genotoxic response of TiO2 nanoparticles and C60 in mammalian cells. In the present study, we determined the mutation fractions induced by either TiO2 nanoparticles or C60 in gpt delta transgenic mouse primary embryo fibroblasts (MEF) and identified peroxynitrite anions (ONOO-) as an essential mediator involved in such process.
resultsBoth TiO2 nanoparticles and C60 dramatically increased the mutation yield, which could be abrogated by concurrent treatment with the endocytosis inhibitor, Nystatin. Under confocal scanning microscopy together with the radical probe dihydrorhodamine 123 (DHR 123), we found that there was a dose-dependent formation of ONOO- in live MEF cells exposed to either TiO2 nanoparticles or C60, and the protective effects of antioxidants were demonstrated by the nitric oxide synthase (NOS) inhibitor, NG-methyl-L-arginine (L-NMMA). Furthermore, suppression of cyclooxygenase-2 (COX-2) activity by using the chemical inhibitor NS-398 significantly reduced mutation frequency of both TiO2 nanoparticles and C60.
conclusionOur results provided novel information that both TiO2 nanoparticles and C60 were taken up by cells and induced kilo-base pair deletion mutations in a transgenic mouse mutation system. The induction of ONOO- may be a critical signaling event for nanoparticle genotoxicity.
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.